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Literature summary extracted from

  • Fujihara, J.; Hieda, Y.; Xue, Y.; Nakagami, N.; Takayama, K.; Kataoka, K.; Takeshita, H.
    One-step purification of mammalian deoxyribonucleases I and differences among pancreas, parotid, and pancreas-parotid (mixed) types based on species- and organ-specific N-linked glycosylation (2006), Biochemistry (Moscow), 71 Suppl 1, S65-70.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.21.1 EDTA complete inhibition at 1 mM Oryctolagus cuniculus
3.1.21.1 EDTA complete ihibition at 1 mM; complete inhibition at 1 mM Rattus norvegicus
3.1.21.1 EDTA complete inhibition at 1 mM Sus scrofa
3.1.21.1 EGTA complete inhibition at 1 mM Oryctolagus cuniculus
3.1.21.1 EGTA complete ihibition at 1 mM; complete inhibition at 1 mM Rattus norvegicus
3.1.21.1 EGTA complete inhibition at 1 mM Sus scrofa

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.21.1 Ca2+ required Rattus norvegicus
3.1.21.1 Ca2+ required Sus scrofa
3.1.21.1 Ca2+ required Oryctolagus cuniculus
3.1.21.1 Co2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Rattus norvegicus
3.1.21.1 Co2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Sus scrofa
3.1.21.1 Co2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Oryctolagus cuniculus
3.1.21.1 Mg2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Rattus norvegicus
3.1.21.1 Mg2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Sus scrofa
3.1.21.1 Mg2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Oryctolagus cuniculus
3.1.21.1 Mn2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Rattus norvegicus
3.1.21.1 Mn2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Sus scrofa
3.1.21.1 Mn2+ 5 mM Mg2+, Mn2+ or Co2+ together with Ca2+ required Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.21.1 32000
-
SDS-PAGE Rattus norvegicus
3.1.21.1 34500
-
1 * 34500, SDS-PAGE, gel filtration Sus scrofa
3.1.21.1 35000
-
SDS-PAGE Oryctolagus cuniculus
3.1.21.1 345000
-
SDS-PAGE Sus scrofa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O Rattus norvegicus
-
5'-phosphooligonucleotides + ?
-
?
3.1.21.1 double-stranded DNA + H2O Sus scrofa
-
5'-phosphooligonucleotides + ?
-
?
3.1.21.1 double-stranded DNA + H2O Oryctolagus cuniculus
-
5'-phosphooligonucleotides + ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.1.21.1 Oryctolagus cuniculus
-
-
-
3.1.21.1 Rattus norvegicus
-
-
-
3.1.21.1 Sus scrofa
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.21.1 glycoprotein
-
Rattus norvegicus
3.1.21.1 glycoprotein
-
Sus scrofa
3.1.21.1 glycoprotein
-
Oryctolagus cuniculus

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.21.1 one-step purification by lectin affinity chromatography using a combined concanavalin A and wheat germ agglutinin column Rattus norvegicus
3.1.21.1 one-step purification by lectin affinity chromatography using a combined concanavalin A and wheat germ agglutinin column Sus scrofa
3.1.21.1 one-step purification by lectin affinity chromatography using a combined concanavalin A and wheat germ agglutinin column Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.21.1 pancreas
-
Sus scrofa
-
3.1.21.1 pancreas
-
Oryctolagus cuniculus
-
3.1.21.1 parotid gland
-
Rattus norvegicus
-
3.1.21.1 parotid gland
-
Oryctolagus cuniculus
-
3.1.21.1 submandibular gland
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.21.1 double-stranded DNA + H2O
-
Rattus norvegicus 5'-phosphooligonucleotides + ?
-
?
3.1.21.1 double-stranded DNA + H2O
-
Sus scrofa 5'-phosphooligonucleotides + ?
-
?
3.1.21.1 double-stranded DNA + H2O
-
Oryctolagus cuniculus 5'-phosphooligonucleotides + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.1.21.1 monomer 1 * 32000, SDS-PAGE, gel filtration Rattus norvegicus
3.1.21.1 monomer 1 * 34500, SDS-PAGE, gel filtration Sus scrofa
3.1.21.1 monomer 1 * 35000, SDS-PAGE, gel filtration Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
3.1.21.1 deoxyribonuclease I
-
Rattus norvegicus
3.1.21.1 deoxyribonuclease I
-
Sus scrofa
3.1.21.1 deoxyribonuclease I
-
Oryctolagus cuniculus
3.1.21.1 DNase I
-
Rattus norvegicus
3.1.21.1 DNase I
-
Sus scrofa
3.1.21.1 DNase I
-
Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.21.1 7
-
-
Rattus norvegicus
3.1.21.1 7
-
-
Sus scrofa
3.1.21.1 7
-
-
Oryctolagus cuniculus