BRENDA - Enzyme Database

Purification and properties of isocitrate lyase from pupas of the butterfly Papilio machaon L

Eprintsev, A.T.; Shevchenko, M.Y.; Popov, V.N.; Biochemistry (Moscow) 69, 376-380 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
additional information
no significant change in enzyme activity after incubation with serine, glycine, aspartic and glutamic acids
Papilio machaon
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
D-fructose-6-phosphate
-
Papilio machaon
4.1.3.1
D-Glucose-6-phosphate
-
Papilio machaon
4.1.3.1
malate
competitive mode
Papilio machaon
4.1.3.1
succinate
competitive mode
Papilio machaon
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
1.4
-
isocitrate
pH 7.5, 25°C
Papilio machaon
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
additional information
no significant change in enzyme activity after incubation with Co2+, Pb2+, Fe2+, Cu2+, Cd2+, Na+, K+, Cl-
Papilio machaon
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
13800
-
gel filtration
Papilio machaon
4.1.3.1
68000
-
2 * 68000, SDS-PAGE
Papilio machaon
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
4.1.3.1
Papilio machaon
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
4.1.3.1
-
Papilio machaon
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
pupa
-
Papilio machaon
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
5.5
-
pH 7.5, 25°C
Papilio machaon
Storage Stability
EC Number
Storage Stability
Organism
4.1.3.1
4°C, 0.1 M Tris-HCl (pH 7.5), 3 mM 2-mercaptoethanol or 6 mM DTT, 5 mM MgCl2, 25% glycerol, 5 days, 10% loss of activity, 30 days, 75% loss of activity
Papilio machaon
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
664147
Papilio machaon
succinate + glyoxylate
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
4.1.3.1
dimer
2 * 68000, SDS-PAGE
Papilio machaon
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
-
Papilio machaon
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.06
-
D-fructose-6-phosphate
-
Papilio machaon
4.1.3.1
0.3
-
D-Glucose-6-phosphate
-
Papilio machaon
4.1.3.1
1
-
malate
-
Papilio machaon
4.1.3.1
1.8
-
succinate
-
Papilio machaon
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
4.1.3.1
additional information
no significant change in enzyme activity after incubation with serine, glycine, aspartic and glutamic acids
Papilio machaon
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
4.1.3.1
D-fructose-6-phosphate
-
Papilio machaon
4.1.3.1
D-Glucose-6-phosphate
-
Papilio machaon
4.1.3.1
malate
competitive mode
Papilio machaon
4.1.3.1
succinate
competitive mode
Papilio machaon
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
4.1.3.1
0.06
-
D-fructose-6-phosphate
-
Papilio machaon
4.1.3.1
0.3
-
D-Glucose-6-phosphate
-
Papilio machaon
4.1.3.1
1
-
malate
-
Papilio machaon
4.1.3.1
1.8
-
succinate
-
Papilio machaon
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
4.1.3.1
1.4
-
isocitrate
pH 7.5, 25°C
Papilio machaon
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
4.1.3.1
additional information
no significant change in enzyme activity after incubation with Co2+, Pb2+, Fe2+, Cu2+, Cd2+, Na+, K+, Cl-
Papilio machaon
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
4.1.3.1
13800
-
gel filtration
Papilio machaon
4.1.3.1
68000
-
2 * 68000, SDS-PAGE
Papilio machaon
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
4.1.3.1
-
Papilio machaon
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
4.1.3.1
pupa
-
Papilio machaon
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
4.1.3.1
5.5
-
pH 7.5, 25°C
Papilio machaon
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
4.1.3.1
4°C, 0.1 M Tris-HCl (pH 7.5), 3 mM 2-mercaptoethanol or 6 mM DTT, 5 mM MgCl2, 25% glycerol, 5 days, 10% loss of activity, 30 days, 75% loss of activity
Papilio machaon
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
4.1.3.1
isocitrate
-
664147
Papilio machaon
succinate + glyoxylate
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
4.1.3.1
dimer
2 * 68000, SDS-PAGE
Papilio machaon
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4.1.3.1
7.5
-
-
Papilio machaon