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Literature summary extracted from
- Purification and properties of isocitrate lyase from pupas of the butterfly Papilio machaon L (2004), Biochemistry (Moscow), 69, 376-380.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | additional information | no significant change in enzyme activity after incubation with serine, glycine, aspartic and glutamic acids | Papilio machaon |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | D-fructose-6-phosphate | - |
Papilio machaon |  |
| 4.1.3.1 | D-Glucose-6-phosphate | - |
Papilio machaon | |
| 4.1.3.1 | malate | competitive mode | Papilio machaon | |
| 4.1.3.1 | succinate | competitive mode | Papilio machaon | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 1.4 | - |
isocitrate | pH 7.5, 25°C | Papilio machaon | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.1.3.1 | additional information | no significant change in enzyme activity after incubation with Co2+, Pb2+, Fe2+, Cu2+, Cd2+, Na+, K+, Cl- | Papilio machaon |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 13800 | - |
gel filtration | Papilio machaon |
| 4.1.3.1 | 68000 | - |
2 * 68000, SDS-PAGE | Papilio machaon |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | Papilio machaon | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.1.3.1 | - |
Papilio machaon |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 4.1.3.1 | pupa | - |
Papilio machaon | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 5.5 | - |
pH 7.5, 25°C | Papilio machaon |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.1.3.1 | 4°C, 0.1 M Tris-HCl (pH 7.5), 3 mM 2-mercaptoethanol or 6 mM DTT, 5 mM MgCl2, 25% glycerol, 5 days, 10% loss of activity, 30 days, 75% loss of activity | Papilio machaon |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.3.1 | isocitrate | - |
Papilio machaon | succinate + glyoxylate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | dimer | 2 * 68000, SDS-PAGE | Papilio machaon |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.3.1 | ICL | - |
Papilio machaon |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.1.3.1 | 7.5 | - |
- |
Papilio machaon |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.3.1 | 0.06 | - |
D-fructose-6-phosphate | - |
Papilio machaon | |
| 4.1.3.1 | 0.3 | - |
D-Glucose-6-phosphate | - |
Papilio machaon | |
| 4.1.3.1 | 1 | - |
malate | - |
Papilio machaon | |
| 4.1.3.1 | 1.8 | - |
succinate | - |
Papilio machaon | |
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