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Literature summary extracted from

  • Wang, Y.K.; Morgan, A.; Stieglitz, K.; Stec, B.; Thompson, B.; Miller, S.J.; Roberts, M.F.
    The temperature dependence of the inositol monophosphatase Km correlates with accumulation of di-myo-inositol 1,1'-phosphate in Archaeoglobus fulgidus (2006), Biochemistry, 45, 3307-3314.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.3.25 additional information the enzyme is not upregulated upon thermal stress Archaeoglobus fulgidus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.3.25 expression in Escherichia coli strain BL21(DE3) Methanocaldococcus jannaschii
3.1.3.25 expression in Escherichia coli strain BL21(DE3) Thermotoga maritima
3.1.3.25 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Archaeoglobus fulgidus

Protein Variants

EC Number Protein Variants Comment Organism
3.1.3.25 S171A site-directed mutagenesis, mutation of an active site residue involved in hydrogen bonding, the mutant shows impaired Mg2+ binding, weak substrate binding at all temperatures, and no change in Km with increasing temperature in contrast to the wild-type enzyme Archaeoglobus fulgidus
3.1.3.25 T174L site-directed mutagenesis, mutation of an active site residue involved in hydrogen bonding, the mutant shows no change in Km with increasing temperature in contrast to the wild-type enzyme Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.3.11 additional information
-
 additional information enzyme shows unusual temperature dependence for its main substrate di-myo-inositol 1,1’-phosphate Archaeoglobus fulgidus
3.1.3.11 0.15
-
 D-fructose 1,6-bisphosphate 70°C Archaeoglobus fulgidus
3.1.3.11 0.15
-
 D-fructose 1,6-bisphosphate 55°C Archaeoglobus fulgidus
3.1.3.25 additional information
-
 additional information analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, kinetic analysis Methanocaldococcus jannaschii
3.1.3.25 additional information
-
 additional information analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, kinetic analysis Thermotoga maritima
3.1.3.25 additional information
-
 additional information analysis of temperature-dependence of the Km value of inositol 1-phosphate substrates, the enzyme's fructose bisphosphatase activity is temperature independent between 55°C and 70°C, but fructose bisphosphate is not stable above 70°C Archaeoglobus fulgidus
3.1.3.25 1.67
-
 L-myo-Inositol 1-phosphate 85°C Archaeoglobus fulgidus
3.1.3.25 13.2
-
 L-myo-Inositol 1-phosphate 75°C Archaeoglobus fulgidus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.3.25 Mg2+
-
 Methanocaldococcus jannaschii
3.1.3.25 Mg2+
-
 Thermotoga maritima
3.1.3.25 Mg2+ dependent on, involved in temeprature-dependent substrate affinity regulation Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.3.25 D-myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus
-
 D-myo-inositol + phosphate
-
 ?
3.1.3.25 L-myo-inositol 1-phosphate + H2O Archaeoglobus fulgidus
-
 L-myo-inositol + phosphate
-
 ?
3.1.3.25 additional information Archaeoglobus fulgidus at temperatures above 80°C the organism accumulates di-myo-inositol 1,1'-phosphate as a compatible solute which is regulated by the temperature dependence of the substrate affinity ?
-
 ?
3.1.3.25 additional information Methanocaldococcus jannaschii the organism does not accumulate di-myo-inositol 1,1'-phosphate at temperatures above 80°C ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.3.11 Archaeoglobus fulgidus
-
 inositol monophosphatase exhibits enzyme activity
-
3.1.3.25 Archaeoglobus fulgidus O30298
-
-
3.1.3.25 Methanocaldococcus jannaschii
-
-
-
3.1.3.25 Thermotoga maritima
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.3.25 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography Methanocaldococcus jannaschii
3.1.3.25 recombinant enzyme from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography Thermotoga maritima
3.1.3.25 recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by heat treatment at 85°C and QFF resin chromatography Archaeoglobus fulgidus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.1.3.25 myo-inositol phosphate + H2O = myo-inositol + phosphate the active site residues Tyr155, Glu175, Asp85, and Ser171 are required for the hydrogen bond network in the active site Archaeoglobus fulgidus
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.3.11 D-fructose 1,6-bisphosphate + H2O
-
 Archaeoglobus fulgidus D-fructose 6-phosphate + phosphate
-
 ?
3.1.3.25 3,5-dideoxy-D-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus 3,5-dideoxy-D-myo-inositol + phosphate
-
 ?
3.1.3.25 3,5-dideoxy-L-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus 3,5-dideoxy-L-myo-inositol + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii D-myo-inositol + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima D-myo-inositol + phosphate
-
 ?
3.1.3.25 D-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus D-myo-inositol + phosphate
-
 ?
3.1.3.25 L-myo-inositol 1-phosphate + H2O
-
 Methanocaldococcus jannaschii L-myo-inositol + phosphate
-
 ?
3.1.3.25 L-myo-inositol 1-phosphate + H2O
-
 Thermotoga maritima L-myo-inositol + phosphate
-
 ?
3.1.3.25 L-myo-inositol 1-phosphate + H2O
-
 Archaeoglobus fulgidus L-myo-inositol + phosphate
-
 ?
3.1.3.25 additional information at temperatures above 80°C the organism accumulates di-myo-inositol 1,1'-phosphate as a compatible solute which is regulated by the temperature dependence of the substrate affinity Archaeoglobus fulgidus ?
-
 ?
3.1.3.25 additional information the organism does not accumulate di-myo-inositol 1,1'-phosphate at temperatures above 80°C Methanocaldococcus jannaschii ?
-
 ?
3.1.3.25 additional information the enzyme also shows low fructose bisphosphatase activity Archaeoglobus fulgidus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.3.25 dimer
-
 Methanocaldococcus jannaschii
3.1.3.25 dimer
-
 Archaeoglobus fulgidus
3.1.3.25 tetramer
-
 Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.1.3.25 IMPase
-
 Methanocaldococcus jannaschii
3.1.3.25 IMPase
-
 Thermotoga maritima
3.1.3.25 IMPase
-
 Archaeoglobus fulgidus
3.1.3.25 inositol monophosphatase
-
 Methanocaldococcus jannaschii
3.1.3.25 inositol monophosphatase
-
 Thermotoga maritima
3.1.3.25 inositol monophosphatase
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.3.25 70
-
 assay at Methanocaldococcus jannaschii
3.1.3.25 70
-
 assay at Thermotoga maritima
3.1.3.25 85
-
 or above Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.1.3.25 55 85 the activity increases with increasing temperature, temperature profile Archaeoglobus fulgidus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.1.3.25 additional information
-
 3,5-dideoxy-L-myo-inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
3.1.3.25 additional information
-
 additional information values extrapolated from Arrhenius plot Thermotoga maritima
3.1.3.25 2.49
-
 3,5-dideoxy-D-myo-inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
3.1.3.25 3.64
-
 L-myo-Inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
3.1.3.25 4.32
-
 D-myo-Inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
3.1.3.25 5.78
-
 3,5-dideoxy-L-myo-inositol 1-phosphate pH 8.0, 85°C Archaeoglobus fulgidus
3.1.3.25 7.72
-
 D-myo-Inositol 1-phosphate pH 8.0, 85°C Methanocaldococcus jannaschii
3.1.3.25 7.92
-
 L-myo-Inositol 1-phosphate pH 8.0, 85°C Methanocaldococcus jannaschii
3.1.3.25 16.6
-
 L-myo-Inositol 1-phosphate pH 8.0, 85°C Thermotoga maritima
3.1.3.25 104
-
 D-myo-Inositol 1-phosphate pH 8.0, 85°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.3.25 8
-
 assay at Methanocaldococcus jannaschii
3.1.3.25 8
-
 assay at Thermotoga maritima
3.1.3.25 8
-
 assay at Archaeoglobus fulgidus