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Literature summary extracted from

  • Tsai, M.; Koo, J.; Howell, P.L.
    Recovery of argininosuccinate lyase activity in duck delta1 crystallin (2005), Biochemistry, 44, 9034-9044.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.1 expressed as His tag enzymes Anas sp.

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.1 A41G/I43M mutant without enzyme activity Anas sp.
4.3.2.1 D115A mutant with 45% enzyme activity Anas sp.
4.3.2.1 D115E mutant with 42.6% enzyme activity Anas sp.
4.3.2.1 E115D mutant without enzyme activity Anas sp.
4.3.2.1 I143M mutant without enzyme activity Anas sp.
4.3.2.1 M9F/E115D mutant with 3.3% enzyme activity Anas sp.
4.3.2.1 M9W mutant with 2.0% enzyme activity Anas sp.
4.3.2.1 M9W/E115D mutant with 17% enzyme activity Anas sp.
4.3.2.1 M9W/V14S mutant with 1.9% enzyme activity Anas sp.
4.3.2.1 M9Y/E115D mutant with 5.5% enzyme activity Anas sp.
4.3.2.1 TRUN1-8 mutant with 37% enzyme activity Anas sp.
4.3.2.1 TRUN1-9 mutant with 2.1% enzyme activity Anas sp.
4.3.2.1 W9A mutant with 1.9% enzyme activity Anas sp.
4.3.2.1 W9F mutant with 11.3% enzyme activity Anas sp.
4.3.2.1 W9M mutant with 1.0% enzyme activity Anas sp.
4.3.2.1 W9R mutant with 3% enzyme activity Anas sp.
4.3.2.1 W9Y mutant with 49.5% enzyme activity Anas sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.1 0.045
-
 argininosuccinate wild type, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.069
-
 argininosuccinate mutant W9R, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.083
-
 argininosuccinate mutant TRUN1-8, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.105
-
 argininosuccinate mutant TRUN1-9, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.106
-
 argininosuccinate mutant W9Y, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.111
-
 argininosuccinate mutant W9F, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.112
-
 argininosuccinate mutant W9A, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.115
-
 argininosuccinate mutant W9M, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.119
-
 argininosuccinate 211-chimera, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.212
-
 argininosuccinate mutant M9F/E115D, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.255
-
 argininosuccinate mutant D115A, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.273
-
 argininosuccinate mutant D115E, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.294
-
 argininosuccinate mutant M9W/E115D, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 0.61
-
 argininosuccinate mutant M9Y/E115D, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 2.724
-
 argininosuccinate mutant M9W, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.
4.3.2.1 3.159
-
 argininosuccinate mutant M9W/V14S, 25 °C, 10 mM Tris-HCl, pH 7.5, 1 mM EDTA Anas sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.1 omega-N-(L-arginino)succinate Anas sp.
-
 L-arginine + fumarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.1 Anas sp.
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.1
-
 Anas sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.3.2.1 eye
-
 Anas sp.
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.1 argininosuccinate
-
 Anas sp. fumarate + L-arginine
-
 ?
4.3.2.1 omega-N-(L-arginino)succinate
-
 Anas sp. L-arginine + fumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.1 homotetramer
-
 Anas sp.

Synonyms

EC Number Synonyms Comment Organism
4.3.2.1 ASL delta2-crystallin Anas sp.