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Literature summary extracted from

  • Katsemi, V.; Lucke, C.; Koepke, J.; Lohr, F.; Maurer, S.; Fritzsch, G.; Ruterjans, H.
    Mutational and structural studies of the diisopropylfluorophosphatase from Loligo vulgaris shed new light on the catalytic mechanism of the enzyme (2005), Biochemistry, 44, 9022-9033.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.8.2 expression in Escherichia coli Loligo vulgaris

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.1.8.2 wild-type and mutant N175D, 1.7 A resolution Loligo vulgaris

Protein Variants

EC Number Protein Variants Comment Organism
3.1.8.2 D121F no activity Loligo vulgaris
3.1.8.2 D229N/N120D no activity Loligo vulgaris
3.1.8.2 D232S 3% higher activity than the wild-type Loligo vulgaris
3.1.8.2 F173A 84% lower activity than the wild-type Loligo vulgaris
3.1.8.2 F173L 28% lower activity than the wild-type Loligo vulgaris
3.1.8.2 F173S 68% lower activity than the wild-type Loligo vulgaris
3.1.8.2 F173V 46% lower activity than the wild-type Loligo vulgaris
3.1.8.2 F173W 19% lower activity than the wild-type Loligo vulgaris
3.1.8.2 F173Y 53% lower activity than the wild-type Loligo vulgaris
3.1.8.2 F314A 3% higher activity than the wild-type Loligo vulgaris
3.1.8.2 H181N 19% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H274N 7% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287A 90% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287D 99% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287F 36% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287L 21% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287N 96% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287Q 54% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287W 34% lower activity than the wild-type Loligo vulgaris
3.1.8.2 H287Y 57% lower activity than the wild-type Loligo vulgaris
3.1.8.2 M148A 26% lower activity than the wild-type Loligo vulgaris
3.1.8.2 N120D 96% lower activity than the wild-type Loligo vulgaris
3.1.8.2 N175D 98% lower activity than the wild-type Loligo vulgaris
3.1.8.2 N237S 4% lower activity than the wild-type Loligo vulgaris
3.1.8.2 N272F no activity Loligo vulgaris
3.1.8.2 Q304F 50% lower activity than the wild-type Loligo vulgaris
3.1.8.2 Q304W 3% lower activity than the wild-type Loligo vulgaris
3.1.8.2 Q77F no activity Loligo vulgaris
3.1.8.2 Q77W 6% higher activity than the wild-type Loligo vulgaris
3.1.8.2 Q77Y 6% lower activity than the wild-type Loligo vulgaris
3.1.8.2 R146S 45% lower activity than the wild-type Loligo vulgaris
3.1.8.2 S271A 34% higher activity than the wild-type Loligo vulgaris
3.1.8.2 S271A/D232S 19% lower activity than the wild-type Loligo vulgaris
3.1.8.2 T195A 60% lower activity than the wild-type Loligo vulgaris
3.1.8.2 T195L 11% lower activity than the wild-type Loligo vulgaris
3.1.8.2 T195V 3% lower activity than the wild-type Loligo vulgaris
3.1.8.2 Y144S 8% higher activity than the wild-type Loligo vulgaris

Organism

EC Number Organism UniProt Comment Textmining
3.1.8.2 Loligo vulgaris Q7SIG4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.8.2
-
 Loligo vulgaris

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.8.2 4
-
 mutant N175D Loligo vulgaris
3.1.8.2 7
-
 mutant N120D Loligo vulgaris
3.1.8.2 19
-
 mutant H287A Loligo vulgaris
3.1.8.2 31
-
 mutant F173A Loligo vulgaris
3.1.8.2 63
-
 mutant F173S Loligo vulgaris
3.1.8.2 78
-
 mutant T195A Loligo vulgaris
3.1.8.2 84
-
 mutant H287Y Loligo vulgaris
3.1.8.2 90
-
 mutant H287Q Loligo vulgaris
3.1.8.2 92
-
 mutant F173Y Loligo vulgaris
3.1.8.2 97
-
 mutant Q304F Loligo vulgaris
3.1.8.2 105
-
 mutant F173V Loligo vulgaris
3.1.8.2 106
-
 mutant R146S Loligo vulgaris
3.1.8.2 124
-
 mutant H287F Loligo vulgaris
3.1.8.2 129
-
 mutant H287W Loligo vulgaris
3.1.8.2 143
-
 mutant F173L Loligo vulgaris
3.1.8.2 143
-
 mutant M148A Loligo vulgaris
3.1.8.2 154
-
 mutant H287L Loligo vulgaris
3.1.8.2 158
-
 mutant F173W Loligo vulgaris
3.1.8.2 158
-
 mutant S271A/D232S Loligo vulgaris
3.1.8.2 173
-
 mutant T195L Loligo vulgaris
3.1.8.2 174
-
 mutant Q77Y Loligo vulgaris
3.1.8.2 187
-
 mutant N237S Loligo vulgaris
3.1.8.2 188
-
 mutant Q304W Loligo vulgaris
3.1.8.2 188
-
 mutant T195V Loligo vulgaris
3.1.8.2 193
-
 mutant Q77W Loligo vulgaris
3.1.8.2 194
-
 wild-type Loligo vulgaris
3.1.8.2 199
-
 mutant F314A Loligo vulgaris
3.1.8.2 200
-
 mutant D232S Loligo vulgaris
3.1.8.2 209
-
 mutant Y144S Loligo vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.8.2 diisopropyl fluorophosphate + H2O room temperature, pH 7.5 Loligo vulgaris diisopropyl phosphate + fluoride
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.8.2 DFPase
-
 Loligo vulgaris
3.1.8.2 diisopropylfluorophosphatase
-
 Loligo vulgaris