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Literature summary extracted from
- Active site plasticity of endonuclease V from Salmonella typhimurium (2005), Biochemistry, 44, 675-683.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.1.25.1 | gene endo V, expression of wild-type and mutant C-terminally His-tagged enzymes in Escherichia coli strain BL21(DE3) | Salmonella enterica subsp. enterica serovar Typhimurium |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 3.1.25.1 | D35A | site-directed mutagenesis, the mutant shows no deoxyinosine cleavage activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.25.1 | Ca2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium |  |
| 3.1.25.1 | Mg2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.1.25.1 | Mn2+ | activates | Salmonella enterica subsp. enterica serovar Typhimurium | |
| 3.1.25.1 | additional information | the enzyme requires a metal ion for activity | Salmonella enterica subsp. enterica serovar Typhimurium |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.25.1 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | ? | - |
? | |
| 3.1.25.1 | additional information | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.21.7 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
- |
- |
| 3.1.25.1 | Salmonella enterica subsp. enterica serovar Typhimurium | - |
gene endo V | - |
| 3.1.25.1 | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | - |
gene endo V | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.25.1 | recombinant wild-type and mutant C-terminally His-tagged enzymes from Escherichia coli strain BL21(DE3) by nickel chelating affinity chromatography to homogeneity | Salmonella enterica subsp. enterica serovar Typhimurium |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.21.7 | DNA + H2O | limited turnover on cleavage of deoxyinosine- and xanthosine-containing DNA. Nicking activity is similar between the double-stranded deoxyinosine- and deoxyxanthosine-containing DNA. Endonuclease V can only turnover deoxyuridine-containing DNA to a limited extent when substrate is in excess. Endonuclease V achieves tight binding to deoxyuridine-containing DNA. The active site of salmonella endonuclease V can accomodate pyrimidine-containing mismatches, resulting in more comparable cleavage of pyrimidine- and purine-containing mismatches. The plastic nature of the active site allows the enzyme to enfold both purine and pyrimidine deaminated lesions or base pair mismatches | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.1.21.7 | DNA + H2O | limited turnover on cleavage of deoxyinosine- and xanthosine-containing DNA. Nicking activity is similar between the double-stranded deoxyinosine- and deoxyxanthosine-containing DNA. Endonuclease V can only turnover deoxyuridine-containing DNA to a limited extent when substrate is in excess. Endonuclease V achieves tight binding to deoxyuridine-containing DNA. The active site of salmonella endonuclease V can accomodate pyrimidine-containing mismatches, resulting in more comparable cleavage of pyrimidine- and purine-containing mismatches. The plastic nature of the active site allows the enzyme to enfold both purine and pyrimidine deaminated lesions or base pair mismatches | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
| 3.1.25.1 | additional information | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.1.25.1 | additional information | substrate specificity, the enzyme performs single-turnover on cleavage of double-stranded or single-stranded deoxynucleotide-containing DNA, the enzyme cleaves deoxyinosine- or deoxyxanthosine-containing DNA with similar nicking activity for the substrates, tight binding to nicking products, deoxyuridine-containing DNA is only slightly acepted as substrate when it is in excess, the active site of the enzyme can accomodate pyrimidine-containing mismatches, the plastic nature of the active site allows the enzyme to enfold both pyrimidine and purine deaminated lesions or base pair mismatches, overview | Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.1.25.1 | additional information | the enzyme initiates repair of deaminated bases in damaged DNA by making a nucleolytic incision one nucleotide away from the 3' side of the lesion | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
| 3.1.25.1 | additional information | substrate specificity, the enzyme performs single-turnover on cleavage of double-stranded or single-stranded deoxynucleotide-containing DNA, the enzyme cleaves deoxyinosine- or deoxyxanthosine-containing DNA with similar nicking activity for the substrates, tight binding to nicking products, deoxyuridine-containing DNA is only slightly acepted as substrate when it is in excess, the active site of the enzyme can accomodate pyrimidine-containing mismatches, the plastic nature of the active site allows the enzyme to enfold both pyrimidine and purine deaminated lesions or base pair mismatches, overview | Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
| 3.1.25.1 | oligonucleotide DNA + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium | ? | - |
? | |
| 3.1.25.1 | oligonucleotide DNA + H2O | - |
Salmonella enterica subsp. enterica serovar Typhimurium 14028 | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.21.7 | endonuclease V | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.1.25.1 | endonuclease V | - |
Salmonella enterica subsp. enterica serovar Typhimurium |
| 3.1.25.1 | More | the enzyme belongs to the endonuclease V family | Salmonella enterica subsp. enterica serovar Typhimurium |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.25.1 | 37 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.1.25.1 | 7.4 | - |
assay at | Salmonella enterica subsp. enterica serovar Typhimurium |
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