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Literature summary extracted from
- Intermediates and transition states in thiamin diphosphate-dependent decarboxylases. A kinetic and NMR study on wild-type indolepyruvate decarboxylase and variants using indolepyruvate, benzoylformate, and pyruvate as substrates (2005), Biochemistry, 44, 6164-6179.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.1.1.74 | D29E | significant decrease in Km-values | Enterobacter cloacae |
| 4.1.1.74 | E468D | slight decrease in Km-values | Enterobacter cloacae |
| 4.1.1.74 | E52D | decrease in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, increase in Km-value for benzoylformic acid | Enterobacter cloacae |
| 4.1.1.74 | H115K | slight increase in Km-value for 3-(indol-3-yl)pyruvate and pyruvate, decrease in Km-value for benzoylformic acid | Enterobacter cloacae |
| 4.1.1.74 | Q383T | decrease in Km-value for pyruvate | Enterobacter cloacae |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.74 | 0.0045 | - |
3-(Indol-3-yl)pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae |  |
| 4.1.1.74 | 0.018 | - |
3-(Indol-3-yl)pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.02 | - |
3-(Indol-3-yl)pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.024 | - |
benzoylformic acid | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.024 | - |
3-(Indol-3-yl)pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.032 | - |
3-(Indol-3-yl)pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.14 | - |
benzoylformic acid | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.27 | - |
benzoylformic acid | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.63 | - |
pyruvate | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.73 | - |
pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.77 | - |
pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 1.65 | - |
benzoylformic acid | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 1.71 | - |
benzoylformic acid | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 2.72 | - |
benzoylformic acid | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 3.16 | - |
pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 3.38 | - |
pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 3.48 | - |
pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.1.1.74 | Enterobacter cloacae | - |
- |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.1.1.74 | 3-(indol-3-yl)pyruvate = 2-(indol-3-yl)acetaldehyde + CO2 | residues E468, D29, and H115 are involved in binding of all substrates and decarboxlation of the respective tetrahedral thiamine-diphosphate-substrate adducts. Residue E383 is central to substrate specificity | Enterobacter cloacae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.1.1.74 | 3-(indol-3-yl)pyruvate | - |
Enterobacter cloacae | 2-(indol-3-yl)acetaldehyde + CO2 | - |
? | |
| 4.1.1.74 | benzoylformic acid | - |
Enterobacter cloacae | benzaldehyde + CO2 | - |
? | |
| 4.1.1.74 | pyruvate | - |
Enterobacter cloacae | acetaldehyde + CO2 | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 4.1.1.74 | Ipyr | - |
Enterobacter cloacae |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.1.1.74 | 0.01 | - |
benzoylformic acid | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.016 | - |
pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.019 | - |
pyruvate | mutant D29E, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.04 | - |
3-(Indol-3-yl)pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.06 | - |
pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.069 | - |
pyruvate | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.07 | - |
benzoylformic acid | mutant E468D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.1 | - |
3-(Indol-3-yl)pyruvate | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.29 | - |
benzoylformic acid | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.31 | - |
3-(Indol-3-yl)pyruvate | mutant H115K, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.87 | - |
benzoylformic acid | mutant E52D, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.88 | - |
pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 0.98 | - |
3-(Indol-3-yl)pyruvate | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 2.05 | - |
3-(Indol-3-yl)pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 2.06 | - |
pyruvate | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 2.25 | - |
benzoylformic acid | mutant Q383T, pH 6.5, 30°C | Enterobacter cloacae | |
| 4.1.1.74 | 11.6 | - |
benzoylformic acid | wild-type, pH 6.5, 30°C | Enterobacter cloacae | |
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