Literature summary extracted from
Winger, J.A.; Marletta, M.A.
Expression and characterization of the catalytic domains of soluble guanylate cyclase: interaction with the heme domain (2005), Biochemistry, 44, 4083-4090.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
NO |
- |
Rattus norvegicus |
|
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
4.6.1.2 |
catalytic domains (alphacat and betacat) of alpha1beta1 soluble guanylate cyclase are expressed in Escherichia coli |
Rattus norvegicus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
additional information |
the N-terminal heme-bound regulatory domain of the beta1 subunit of soluble guanylate cyclase inhibits the activity of the alphacatbetacat complex in trans, suggesting a domain-scale mechanism of regulation by NO |
Rattus norvegicus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
0.085 |
- |
GTP |
Mn2+-GTP |
Rattus norvegicus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.6.1.2 |
Mg2+ |
physiological cofactor. Like the full-length enzyme, the alphacatbetacat complex is more active in presence of Mn2+ as compared to the physiological cofactor |
Rattus norvegicus |
|
4.6.1.2 |
Mn2+ |
like the full-length enzyme, the alphacatbetacat complex is more active in presence of Mn2+ as compared to the physiological cofactor |
Rattus norvegicus |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.6.1.2 |
Rattus norvegicus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.6.1.2 |
- |
Rattus norvegicus |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
4.6.1.2 |
lung |
- |
Rattus norvegicus |
- |
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.6.1.2 |
More |
each of the catalytic domains alphacat and betacat (expressed in Escherichia coli), form homodimers. Heterodimers are formed when alphacat and betacat are combined |
Rattus norvegicus |