Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Rajan, R.; Zhu, J.; Hu, X.; Pei, D.; Bell, C.E.
    Crystal structure of S-ribosylhomocysteinase (LuxS) in complex with a catalytic 2-ketone intermediate (2005), Biochemistry, 44, 3745-3753.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.4.1.21 hanging drop vapor diffusion, inactive mutant C84A of Co2+-substituted LuxS is cocrystallized with the 2-ketone intermediate and the structure is determined to 1.8 A resolution Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.4.1.21 C85A catalytically inactive mutant Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.21 0.0014
-
 S-ribosylhomocysteine
-
 Bacillus subtilis
4.4.1.21 0.039
-
 S-ribosylhomocysteine
-
 Vibrio harveyi

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.21 Bacillus subtilis O34667
-
-
4.4.1.21 Vibrio harveyi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.21
-
 Vibrio harveyi
4.4.1.21
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.21 S-ribosylhomocysteine
-
 Vibrio harveyi L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?
4.4.1.21 S-ribosylhomocysteine
-
 Bacillus subtilis L-homocysteine + 4,5-dihydroxy-2,3-pentanedione
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.4.1.21 0.03
-
 S-ribosylhomocysteine
-
 Bacillus subtilis
4.4.1.21 0.4
-
 S-ribosylhomocysteine
-
 Vibrio harveyi