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Literature summary extracted from

  • Wang, J.; Shen, W.J.; Patel, S.; Harada, K.; Kraemer, F.B.
    Mutational analysis of the "regulatory module" of hormone-sensitive lipase (2005), Biochemistry, 44, 1953-1959.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.79 DELTA644-658 reduced basal hydrolytic activity, no activation by cyclic AMP-dependent protein kinase Rattus norvegicus
3.1.1.79 DELTA657-664 reduced basal hydrolytic activity, no activation by cyclic AMP-dependent protein kinase Rattus norvegicus
3.1.1.79 DELTA666-683 very low basal and stimulated hydrolytic activity. Vmax with cholesteryl ester is 29fold lower than wild-type value. Vmax with p-nitrophenylbutyrate is 8fold lower than wild-type value Rattus norvegicus
3.1.1.79 F650C reduced activity against lipid-soluble but not water-soluble substrates Rattus norvegicus
3.1.1.79 F654D reduced activity against lipid-soluble but not water-soluble substrates Rattus norvegicus
3.1.1.79 P651A reduced activity against lipid-soluble but not water-soluble substrates Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.79 0.015
-
 cholesteryl oleate wild-type enzyme Rattus norvegicus
3.1.1.79 0.021
-
 cholesteryl oleate DELTA666-683 Rattus norvegicus
3.1.1.79 0.024
-
 cholesteryl oleate DELTA644-658 Rattus norvegicus
3.1.1.79 0.025
-
 cholesteryl oleate DELTA657-664 Rattus norvegicus
3.1.1.79 0.268
-
 4-nitrophenyl oleate DELTA666-683 Rattus norvegicus
3.1.1.79 0.389
-
 4-nitrophenyl oleate DELTA644-658 Rattus norvegicus
3.1.1.79 0.427
-
 4-nitrophenyl oleate DELTA657-664 Rattus norvegicus
3.1.1.79 0.462
-
 4-nitrophenyl oleate wild-type enzyme Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.79 additional information Rattus norvegicus rate-limiting enzyme in lipolysis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.79 Rattus norvegicus
-
 recombinant
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.79 4-nitrophenyl oleate + H2O
-
 Rattus norvegicus 4-nitrophenol + oleate
-
 ?
3.1.1.79 cholesteryl oleate + H2O
-
 Rattus norvegicus cholesterol + oleic acid
-
 ?
3.1.1.79 additional information rate-limiting enzyme in lipolysis Rattus norvegicus ?
-
 ?
3.1.1.79 p-nitrophenyl butyrate + H2O
-
 Rattus norvegicus p-nitrophenol + butanoate
-
 ?