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Literature summary extracted from

  • Schneider, B.; Knochel, T.; Darimont, B.; Hennig, M.; Dietrich, S.; Babinger, K.; Kirschner, K.; Sterner, R.
    Role of the N-terminal extension of the (betaalpha)(8)-barrel enzyme indole-3-glycerol phosphate synthase for its fold, stability, and catalytic activity (2005), Biochemistry, 44, 16405-16412.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.1.1.48 N-terminal deletion mutant lacking 26 amino acids, structure of core is unchanged compared to wild-type Saccharolobus solfataricus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.1.48 additional information deletion of N-terminal 25 amino acids, unchanged oligomerzation states and turnover numbers, increase in Km-value for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate, decrease in resistance towards unfolding induced by heat and guanidinium chloride Thermotoga maritima
4.1.1.48 additional information deletion of N-terminal 26 amino acids, unchanged oligomerzation states and turnover numbers, increase in Km-value for 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate, decrease in resistance towards unfolding induced by heat and guanidinium chloride Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.48 0.000025
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type, pH 7.5, 25°C Thermotoga maritima
4.1.1.48 0.00009
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.00069
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate N-terminal deletion mutant lacking 25 amino acids, pH 7.5, 25°C Thermotoga maritima
4.1.1.48 0.0099
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate N-terminal deletion mutant lacking 26 amino acids, pH 7.5, 25°C Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.48 23500
-
 gel filtration, N-terminal deletion mutant lacking 26 amino acids Saccharolobus solfataricus
4.1.1.48 24200
-
 gel filtration, N-terminal deletion mutant lacking 25 amino acids Thermotoga maritima
4.1.1.48 26300
-
 gel filtration, wild-type Saccharolobus solfataricus
4.1.1.48 26400
-
 1 * 26400, N-terminal deletion mutant lacking 26 amino acids, 1 * 28600, wild-type, calculated Saccharolobus solfataricus
4.1.1.48 26600
-
 and dimer, 1 * 26600, N-terminal deletion mutant lacking 25 amino acids, 1 * 28700, wild-type, calculated Thermotoga maritima
4.1.1.48 26600
-
 and dimer, 2 * 26600, N-terminal deletion mutant lacking 25 amino acids, 2 * 28700, wild-type, calculated Thermotoga maritima
4.1.1.48 28200
-
 gel filtration, wild-type Thermotoga maritima
4.1.1.48 28600
-
 1 * 26400, N-terminal deletion mutant lacking 26 amino acids, 1 * 28600, wild-type, calculated Saccharolobus solfataricus
4.1.1.48 28700
-
 and dimer, 1 * 26600, N-terminal deletion mutant lacking 25 amino acids, 1 * 28700, wild-type, calculated Thermotoga maritima
4.1.1.48 28700
-
 and dimer, 2 * 26600, N-terminal deletion mutant lacking 25 amino acids, 2 * 28700, wild-type, calculated Thermotoga maritima
4.1.1.48 39300
-
 gel filtration, N-terminal deletion mutant lacking 25 amino acids Thermotoga maritima
4.1.1.48 47100
-
 gel filtration, wild-type Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.48 Saccharolobus solfataricus Q06121
-
-
4.1.1.48 Thermotoga maritima Q56319
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Saccharolobus solfataricus 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?
4.1.1.48 1-(2-Carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate
-
 Thermotoga maritima 1-(3-Indolyl)glycerol-3-phosphate + CO2 + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.48 dimer and dimer, 2 * 26600, N-terminal deletion mutant lacking 25 amino acids, 2 * 28700, wild-type, calculated Thermotoga maritima
4.1.1.48 monomer 1 * 26400, N-terminal deletion mutant lacking 26 amino acids, 1 * 28600, wild-type, calculated Saccharolobus solfataricus
4.1.1.48 monomer and dimer, 1 * 26600, N-terminal deletion mutant lacking 25 amino acids, 1 * 28700, wild-type, calculated Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.1.1.48 75
-
 half-life of wild-type, 123 min, half-life of N-terminal deletion mutant lacking 26 amino acids, 2.9 min Saccharolobus solfataricus
4.1.1.48 75
-
 half-life of wild-type, 258 min, half-life of N-terminal deletion mutant lacking 25 amino acids, 6.3 min Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.1.48 0.025
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.028
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate N-terminal deletion mutant lacking 26 amino acids, pH 7.5, 25°C Saccharolobus solfataricus
4.1.1.48 0.06
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate N-terminal deletion mutant lacking 25 amino acids, pH 7.5, 25°C Thermotoga maritima
4.1.1.48 0.095
-
 1-(2-carboxyphenylamino)-1-deoxy-D-ribulose-5-phosphate wild-type, pH 7.5, 25°C Thermotoga maritima