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Literature summary extracted from

  • Lu, Z.; Feng, X.; Song, L.; Han, Y.; Kim, A.; Herzberg, O.; Woodson, W.R.; Martin, B.M.; Mariano, P.S.; Dunaway-Mariano, D.
    Diversity of function in the isocitrate lyase enzyme superfamily: the Dianthus caryophyllus petal death protein cleaves alpha-keto and alpha-hydroxycarboxylic acids (2005), Biochemistry, 44, 16365-16376.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.3.1 expresssion in Escherichia coli Dianthus caryophyllus

Protein Variants

EC Number Protein Variants Comment Organism
4.1.3.1 C144A mutant with no enzyme activity Dianthus caryophyllus
4.1.3.1 D79A mutant with 1000fold reduced turnover rate Dianthus caryophyllus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.3.1 0.026
-
 (2R,3S)-2,3-dimethyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.029
-
 (2R,3S)-2,3-dimethyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.042
-
 (2R,3S)-isocitrate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.069
-
 (2R,3S:2S,3R)-2-methyl-isocitrate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.098
-
 (2R)-propyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.104
-
 (2R,3R:2S,3S)-2-methyl-isocitrate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.13
-
 oxalacetate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.29
-
 (2R)-2-methyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.45
-
 (2R)-ethyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 0.53
-
 (2R)-ethyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 1.1
-
 (2R)-2-ethyl malate pH 7.5, 25°C Dianthus caryophyllus
4.1.3.1 8
-
 (2R)-isobutyl-(3S)-methyl malate pH 7.5, 25°C Dianthus caryophyllus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.3.1 Co2+ activating Dianthus caryophyllus
4.1.3.1 Fe2+ activating Dianthus caryophyllus
4.1.3.1 Mg2+ activating oxalacetate hydrolase activity Dianthus caryophyllus
4.1.3.1 Mn2+ activating oxalacetate hydrolase activity Dianthus caryophyllus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.3.1 35000
-
 4 * 35000, SDS-PAGE Dianthus caryophyllus
4.1.3.1 133000
-
 gel filtration Dianthus caryophyllus

Organism

EC Number Organism UniProt Comment Textmining
4.1.3.1 Dianthus caryophyllus Q05957
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.3.1
-
 Dianthus caryophyllus

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.3.1 (2R)-2-ethyl malate
-
 Dianthus caryophyllus acetate + 2-oxobutanoate
-
 ?
4.1.3.1 (2R)-2-methyl malate
-
 Dianthus caryophyllus pyruvate + acetate
-
 ?
4.1.3.1 (2R)-ethyl-(3S)-methyl malate
-
 Dianthus caryophyllus 2-oxobutanoate + propanoate
-
 ?
4.1.3.1 (2R)-isobutyl-(3S)-methyl malate
-
 Dianthus caryophyllus 4-methyl-2-oxopentanoate + propanoate
-
 ?
4.1.3.1 (2R)-propyl-(3S)-methyl malate
-
 Dianthus caryophyllus 4-methyl-2-oxopentanoate + propanoate
-
 ?
4.1.3.1 (2R,3R:2S,3S)-2-methyl-isocitrate
-
 Dianthus caryophyllus succinate + pyruvate
-
 ?
4.1.3.1 (2R,3S)-2,3-dimethyl malate
-
 Dianthus caryophyllus pyruvate + propanoate
-
 ?
4.1.3.1 (2R,3S)-isocitrate
-
 Dianthus caryophyllus succinate + glyoxylate
-
 ?
4.1.3.1 (2R,3S:2S,3R)-2-methyl-isocitrate
-
 Dianthus caryophyllus succinate + pyruvate
-
 ?
4.1.3.1 (2S)-2-methyl malate kcat below 1x10-5 Dianthus caryophyllus pyruvate + acetate
-
 ?
4.1.3.1 (R)-malate kcat below 1x10-5 Dianthus caryophyllus glyoxylate + acetate
-
 ?
4.1.3.1 (S)-malate kcat below 1x10-5 Dianthus caryophyllus glyoxylate + acetate
-
 ?
4.1.3.1 oxalacetate
-
 Dianthus caryophyllus oxalate + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.3.1 tetramer 4 * 35000, SDS-PAGE Dianthus caryophyllus

Synonyms

EC Number Synonyms Comment Organism
4.1.3.1 PDP
-
 Dianthus caryophyllus
4.1.3.1 petal death protein
-
 Dianthus caryophyllus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.3.1 7
-
 (2R,3S)-2-ethyl-3-methyl malate Dianthus caryophyllus