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Literature summary extracted from

  • Le Nours, J.; Anderson, L.; Stoll, D.; Stalbrand, H.; Lo Leggio, L.
    The structure and characterization of a modular endo-beta-1,4-mannanase from Cellulomonas fimi (2005), Biochemistry, 44, 12700-12708.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.78 expressed in Escherichia coli Cellulomonas fimi

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.78 hanging-drop vapor diffusion method at room temperature Cellulomonas fimi

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.78 cell wall
-
 Cellulomonas fimi 5618
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.78 50000
-
 SDS-PAGE, a stable fragment corresponding to about 460 residues and comprising the catalytic domain and 50 to 60 extra residues at the C-terminus Cellulomonas fimi

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.78 Cellulomonas fimi
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.78 0.69
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man} Cellulomonas fimi
3.2.1.78 1.13
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man Cellulomonas fimi
3.2.1.78 1.95
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man Cellulomonas fimi
3.2.1.78 3.1
-
 Manbeta(1-4)Manbeta(1-4)Man Cellulomonas fimi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi mannose + Manbeta(1-4)Man
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + mannose
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi mannose + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + H2O
-
 Cellulomonas fimi D-mannitol + Manbeta(1-4)Man-ol + D-mannose + Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man-ol + Manbeta(1-4)Manbeta(1-4)Man
-
 ?
3.2.1.78 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + H2O
-
 Cellulomonas fimi Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man + Manbeta(1-4)Man + Manbeta(1-4)Manbeta(1-4)Man + mannose + Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.78 endo-beta-1,4-mannanase
-
 Cellulomonas fimi
3.2.1.78 Man26A-50K
-
 Cellulomonas fimi

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.78 37
-
-
 Cellulomonas fimi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.78 0.26
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
3.2.1.78 0.41
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
3.2.1.78 1.17
-
 Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
3.2.1.78 11.64
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
3.2.1.78 52.42
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi
3.2.1.78 77
-
 Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Manbeta(1-4)Man
-
 Cellulomonas fimi

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.78 6
-
-
 Cellulomonas fimi