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Literature summary extracted from

  • Matsuura, I.; Wang, G.; He, D.; Liu, F.
    Identification and characterization of ERK MAP kinase phosphorylation sites in Smad3 (2005), Biochemistry, 44, 12546-12553.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.24 EGF induces phosphorylation of Smad3 Mus musculus
2.7.11.24 additional information ERK2 is activated by phosphorylation Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.11.24 additional information PD98059 and U0126 inhibit EGF-induced phosphorylation of Smad3 Mus musculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.11.24 Mg2+
-
 Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.11.24 ATP + Smad3 Mus musculus substrate of MAPKs, e.g. ERK2 ADP + phosphorylated Smad3
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.11.24 Mus musculus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.11.24 phosphoprotein ERK2 is activated by phosphorylation Mus musculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.11.24 C2C12 cell
-
 Mus musculus
-
2.7.11.24 myoblast
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.11.24 ATP + Elk1 recombinant GST-tagged Elk1, substrate of ERK2 Mus musculus ADP + phosphorylated Elk1
-
 ?
2.7.11.24 ATP + myelin basic protein substrate of ERK2 Mus musculus ADP + phosphorylated myelin basic protein
-
 ?
2.7.11.24 ATP + Smad3 substrate of MAPKs, e.g. ERK2 Mus musculus ADP + phosphorylated Smad3
-
 ?
2.7.11.24 ATP + Smad3 substrate of MAPKs, e.g. ERK2, identification of phosphorylation sites Ser203, Ser207, and Thr187, Ser207 is the best phosphorylation site for ERK2, other MAPKs than ERK2 also phosphorylate Ser212 Mus musculus ADP + phosphorylated Smad3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.11.24 ERK
-
 Mus musculus
2.7.11.24 ERK2
-
 Mus musculus
2.7.11.24 MAP kinase
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.11.24 30
-
 assay at Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.11.24 7.4
-
 assay at Mus musculus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.11.24 ATP
-
 Mus musculus