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Literature summary extracted from

  • Segall, M.L.; Colman, R.F.
    Gln212, Asn270, and Arg301 are critical for catalysis by adenylosuccinate lyase from Bacillus subtilis (2004), Biochemistry, 43, 7391-7402.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.2.2 expression in Escherichia coli as His tag enzymes Bacillus subtilis

Protein Variants

EC Number Protein Variants Comment Organism
4.3.2.2 N270D mutant with impaired activity Bacillus subtilis
4.3.2.2 N270L mutant with impaired activity Bacillus subtilis
4.3.2.2 Q212E mutant with impaired activity Bacillus subtilis
4.3.2.2 Q212M mutant with impaired activity Bacillus subtilis
4.3.2.2 R301K mutant with impaired activity Bacillus subtilis
4.3.2.2 R301Q mutant with impaired activity Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.3.2.2 0.0027
-
 adenylosuccinate wild type, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis
4.3.2.2 0.0166
-
 adenylsuccinate mutant Q212E, 50 mM HEPES, pH 7.0, 25°C Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.3.2.2 50000
-
 2 * 50000, SDS-PAGE Bacillus subtilis
4.3.2.2 50000
-
 4 * 50000, SDS-PAGE Bacillus subtilis
4.3.2.2 152000
-
 mutant N270L, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
4.3.2.2 153000
-
 wild type, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
4.3.2.2 159000
-
 mutant Q212E, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
4.3.2.2 166000
-
 mutant N270D, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
4.3.2.2 173000
-
 mutant R301K, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
4.3.2.2 174000
-
 mutant Q212M, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis
4.3.2.2 200000
-
 SDS-PAGE Bacillus subtilis
4.3.2.2 219000
-
 mutant R301Q, light scattering measurements, 0.2 mg/ml enzyme in a filtered 20 mM potassium phosphate buffer at pH 7.0, containing 20 mM potassium chloride Bacillus subtilis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide Bacillus subtilis
-
 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 adenylosuccinate Bacillus subtilis
-
 AMP + fumarate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.3.2.2 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.2.2 Ni-affinity chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.2.2 5-aminoimidazole-4-(N-succinylcarboxamide) ribonucleotide
-
 Bacillus subtilis 5-aminoimidazole-4-carboxamide ribonucleotide + fumarate
-
 ?
4.3.2.2 adenylosuccinate
-
 Bacillus subtilis AMP + fumarate
-
 ?

Subunits

EC Number Subunits Comment Organism
4.3.2.2 homodimer 2 * 50000, SDS-PAGE Bacillus subtilis
4.3.2.2 homotetramer 4 * 50000, SDS-PAGE Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
4.3.2.2 ASL
-
 Bacillus subtilis