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Literature summary extracted from

  • Yeh, J.I.; Du, S.; Pohl, E.; Cane, D.E.
    Multistate binding in pyridoxine 5'-phosphate synthase: 1.96 A crystal structure in complex with 1-deoxy-D-xylulose phosphate (2002), Biochemistry, 41, 11649-11657.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.6.99.2 12 mg/ml purified recombinant enzyme, wild-type or selenomethionine-labeled, in 10 mM Tris-HCl, pH 7.5, 0.4 mM potassium phosphate, 1.21 mM NaCl, 8% PEG 8000, 9% PEG 1000, 10% glycerol, and 4 mM 1-deoxy-D-xylulose, hanging drop vapour diffusion method, X-ray diffraction structure determination and analysis at 1.96-3.2 A resolution Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.99.2 additional information
-
additional information thermodynamics Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate Escherichia coli
-
pyridoxine 5'-phosphate + phosphate + H2O
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.6.99.2 Escherichia coli P0A794
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.99.2 recombinant enzyme by hydroxylapatite and ion exchange chromatography, and gel filtration Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate = pyridoxine 5'-phosphate + phosphate + 2 H2O reaction energetics and mechanism, active site structure, substrate binding structure, determination of conformation states of the enzyme during reaction from apo to single occupied transitional binding state and to fully occupied state Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate
-
Escherichia coli pyridoxine 5'-phosphate + phosphate + H2O
-
?
2.6.99.2 1-deoxy-D-xylulose 5-phosphate + 3-amino-2-oxopropyl phosphate 1-deoxy-D-xylulose 5-phosphate binding structure Escherichia coli pyridoxine 5'-phosphate + phosphate + H2O
-
?

Synonyms

EC Number Synonyms Comment Organism
2.6.99.2 PdxJ
-
Escherichia coli