Any feedback?
Literature summary extracted from
- Production, purification, and characterization of a Mg2+-responsive porphobilinogen synthase from pseudomonas aeruginosa (1999), Biochemistry, 38, 13968-13975.
Activating Compound
| EC Number | Activating Compound | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | NH4+ | stimulates | Pseudomonas aeruginosa |  |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 4.2.1.24 | expression in Escherichia coli | Pseudomonas aeruginosa |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 4.2.1.24 | hanging drop vapour diffusion method, crystals of the enzyme complex with levulinic acid solved at 1.67 A resolution, crystals belong to space group P42(1)2 with cell dimensions of a = b = 129.8 A, c = 86.7 A | Pseudomonas aeruginosa |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | 2,2-difluorosuccinic acid | competitive | Pseudomonas aeruginosa | |
| 4.2.1.24 | diethyl dicarbonate | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | EDTA | activity can be completely restored by addition of Mg2+ or Mn2+. Co2+, Zn2+, and Ni2+ partially restore EDTA-inhibited activity | Pseudomonas aeruginosa | |
| 4.2.1.24 | levulinic acid | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | protoporphyrin IX | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | pyridoxal phosphate | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | succinic acid | noncompetitive | Pseudomonas aeruginosa | |
| 4.2.1.24 | succinic acid monomethyl ester | competitive | Pseudomonas aeruginosa | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.24 | 0.33 | - |
5-aminolevulinate | pH 8.6 in Na-Hepes buffer | Pseudomonas aeruginosa | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.2.1.24 | Co2+ | partially restores activity after inhibition with EDTA | Pseudomonas aeruginosa | |
| 4.2.1.24 | K+ | stimulates | Pseudomonas aeruginosa | |
| 4.2.1.24 | Li+ | stimulates | Pseudomonas aeruginosa | |
| 4.2.1.24 | Mg2+ | can completrly restore activity after inhibition by EDTA | Pseudomonas aeruginosa | |
| 4.2.1.24 | Mg2+ | can completrly restore activity after inhibition by EDTA, stabilizes the oligomeric state but is not essential for octamer formation | Pseudomonas aeruginosa | |
| 4.2.1.24 | Na+ | stimulates | Pseudomonas aeruginosa | |
| 4.2.1.24 | Ni2+ | partially restores activity after inhibition with EDTA | Pseudomonas aeruginosa | |
| 4.2.1.24 | Zn2+ | partially restores activity after inhibition with EDTA | Pseudomonas aeruginosa | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 37832 | - |
6 * 37832, electrospray ionization mass spectrometry | Pseudomonas aeruginosa |
| 4.2.1.24 | 280000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.2.1.24 | Pseudomonas aeruginosa | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 4.2.1.24 | - |
Pseudomonas aeruginosa |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 1 | - |
- |
Pseudomonas aeruginosa |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.2.1.24 | 5-aminolevulinate + 5-aminolevulinate | - |
Pseudomonas aeruginosa | porphobilinogen + 2 H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.2.1.24 | octamer | 6 * 37832, electrospray ionization mass spectrometry | Pseudomonas aeruginosa |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 8.6 | - |
in presence of Mg2+ and K+ | Pseudomonas aeruginosa |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.2.1.24 | 7.5 | 9.8 | pH 7.5: about 35% of maximal activity, pH 9.8: about 50% of maximal activity | Pseudomonas aeruginosa |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.2.1.24 | 0.0025 | - |
protoporphyrin IX | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | 0.03 | - |
diethyl dicarbonate | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | 0.03 | - |
pyridoxal phosphate | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | 1.5 | - |
levulinic acid | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | 1.9 | - |
succinic acid monomethyl ester | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | 7 | - |
2,2-difluorosuccinic acid | - |
Pseudomonas aeruginosa | |
| 4.2.1.24 | 9 | - |
succinic acid | - |
Pseudomonas aeruginosa | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 4.2.1.24 | Pseudomonas aeruginosa | isoelectric focusing, pH-range 3-10 | - |
5.4 |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
