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Literature summary extracted from
- Thermostability enhancement and change in starch hydrolysis profile of the maltohexaose-forming amylase of Bacillus stearothermophilus US100 strain (2006), Biochem. J., 394, 51-56.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.98 | expressed in Escherichia coli | Geobacillus stearothermophilus |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.98 | Geobacillus stearothermophilus | - |
- |
- |
| 3.2.1.98 | Geobacillus stearothermophilus US100 | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.98 | affinity chromatography using the IMPACT-CN system | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.98 | starch + H2O | - |
Geobacillus stearothermophilus | maltohexaose + maltopentaose | - |
? |  |
| 3.2.1.98 | starch + H2O | - |
Geobacillus stearothermophilus US100 | maltohexaose + maltopentaose | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.98 | alpha-amylase | - |
Geobacillus stearothermophilus |
| 3.2.1.98 | Amy | - |
Geobacillus stearothermophilus |
| 3.2.1.98 | maltohexaose-forming amylase | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.98 | 80 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.98 | 100 | - |
mutant protein AmyUS100-IG missing Ile214-Gly215 residues, 70 min stable in presence of 25 mM CaCl2 | Geobacillus stearothermophilus |
| 3.2.1.98 | 100 | - |
wild-type Bacillus Amy, 15 min stable in presence of 100 mM CaCl2 | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.98 | 5.6 | - |
- |
Geobacillus stearothermophilus |
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Release 2023.1 (January 2023)
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