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Literature summary extracted from

  • Wu, J.; Nilsson, A.; Jonsson, B.A.; Stenstad, H.; Agace, W.; Cheng, Y.; Duan, R.D.
    Intestinal alkaline sphingomyelinase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity (2006), Biochem. J., 394, 299-308.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.4.12 bile salt dependent on, particularly taurocholate and taurochenodeoxycholate Homo sapiens
3.1.4.12 bile salt dependent on, particularly taurocholate and taurochenodeoxycholate Rattus norvegicus
3.1.4.12 taurochenodeoxycholate
-
 Homo sapiens
3.1.4.12 taurochenodeoxycholate
-
 Rattus norvegicus
3.1.4.12 taurochenodeoxycholate stimulates hydrolysis of sphingomyelin and platelet-activating factor Rattus norvegicus
3.1.4.12 taurocholate
-
 Homo sapiens
3.1.4.12 taurocholate
-
 Rattus norvegicus
3.1.4.12 taurocholate stimulates hydrolysis of sphingomyelin and platelet-activating factor Rattus norvegicus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.4.12 alk-SMase, expression of wild-type and mutant alk-Smase in COS-7 cells Homo sapiens
3.1.4.12 expression in COS-7 cells Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
3.1.4.12 D199A mutation abolishes hydrolysis of platelet-activating factor Rattus norvegicus
3.1.4.12 D199A mutation of the metal binding site of alk-SMase abolishes the enzyme activity Homo sapiens
3.1.4.12 D246A mutation abolishes hydrolysis of platelet-activating factor Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.12 CHAPS hydrolysis of sphingomyelin and platelet-activating factor Rattus norvegicus
3.1.4.12 EDTA alk-SMase activity with platelet-inactivating factor Homo sapiens
3.1.4.12 EDTA alk-SMase activity with platelet-inactivating factor; inhibits hydrolysis of platelet-activating factor, no effect on hydrolysis of sphingomyelin Rattus norvegicus
3.1.4.12 platelet-activating factor hydrolysis of sphingomyelin Rattus norvegicus
3.1.4.12 platelet-inactivating factor competitive inhibition of sphingomyelin hydrolysis Homo sapiens
3.1.4.12 platelet-inactivating factor competitive inhibition of sphingomyelin hydrolysis Rattus norvegicus
3.1.4.12 sphingomyelin strong competitive inhibition of platelet-inactivating factor hydrolysis Homo sapiens
3.1.4.12 sphingomyelin hydrolysis of platelet-activating factor; strong competitive inhibition of platelet-inactivating factor hydrolysis Rattus norvegicus
3.1.4.12 Triton X-100 hydrolysis of sphingomyelin and platelet-activating factor Rattus norvegicus
3.1.4.12 Zn2+ 0.1-0.25 mM inhibits hydrolysis of platelet-activating factor, concentrations above 0.25 mM inhibit hydrolysis of sphingomyelin and platelet-activating factor Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.12 0.0136
-
 platelet-activating factor
-
 Rattus norvegicus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.4.12 Zn2+ 0.1-0.25 mM stimulates hydrolysis of platelet-activating factor Rattus norvegicus
3.1.4.12 Zn2+ activates the alk-SMase activity with platelet-inactivating factor, mutation of the metal binding site abolishes the enzyme activity Homo sapiens
3.1.4.12 Zn2+ activates the alk-SMase activity with platelet-inactivating factor, mutation of the metal binding site of alk-SMase abolishes the enzyme activity Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.4.12 platelet-activating factor + H2O Homo sapiens the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
-
 ?
3.1.4.12 platelet-activating factor + H2O Rattus norvegicus the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
-
 ?
3.1.4.12 platelet-inactivating factor + H2O Homo sapiens alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
-
 ?
3.1.4.12 platelet-inactivating factor + H2O Rattus norvegicus alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
-
 ?
3.1.4.12 sphingomyelin + H2O Rattus norvegicus intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, and hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O Homo sapiens intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview N-acylsphingosine + choline phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.12 Homo sapiens
-
-
-
3.1.4.12 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.12 recombinant alk-SMase from COS-7 cells Rattus norvegicus
3.1.4.12 recombinant wild-type and mutant alk-Smase from COS-7 cells Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.12 HT-29 cell
-
 Homo sapiens
-
3.1.4.12 intestine
-
 Homo sapiens
-
3.1.4.12 intestine
-
 Rattus norvegicus
-
3.1.4.12 leukocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.12 additional information
-
-
 Homo sapiens
3.1.4.12 additional information
-
-
 Rattus norvegicus
3.1.4.12 6.23
-
 purified enzyme with substrate platelet-inactivating factor Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.12 platelet-activating factor + H2O the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer Homo sapiens 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
-
 ?
3.1.4.12 platelet-activating factor + H2O the enzyme cleaves the phosphocholine head group from platelet-activating factor. Potential protective effect of the enzyme against inflammatory bowel disease and colon cancer Rattus norvegicus 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
-
 ?
3.1.4.12 platelet-activating factor + H2O the enzyme cleaves the phosphocholine head group from platelet-activating factor Homo sapiens 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
-
 ?
3.1.4.12 platelet-activating factor + H2O the enzyme cleaves the phosphocholine head group from platelet-activating factor Rattus norvegicus 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate
-
 ?
3.1.4.12 platelet-inactivating factor + H2O
-
 Homo sapiens 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
-
 ?
3.1.4.12 platelet-inactivating factor + H2O
-
 Rattus norvegicus 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
-
 ?
3.1.4.12 platelet-inactivating factor + H2O alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol Homo sapiens 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
-
 ?
3.1.4.12 platelet-inactivating factor + H2O alk-SMase hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, alk-SMase cleaves the phosphocholine head group from PAF and generates 1-O-alkyl-2-acetyl-sn-glycerol Rattus norvegicus 1-O-alkyl-2-acetyl-sn-glycerol + choline phosphate + ?
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Homo sapiens N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O
-
 Rattus norvegicus N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, and hydrolyses and inactivates platelet-activating factor by a phospholipase C activity, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview Rattus norvegicus N-acylsphingosine + choline phosphate
-
 ?
3.1.4.12 sphingomyelin + H2O intestinal alkaline sphingomyelinase hydrolyses sphingomyelin to generate ceramide in the intestinal tract, it may protect the intestinal mucosa from inflammation and tumorigenesis, overview Homo sapiens N-acylsphingosine + choline phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.1.4.12 alk-SMase
-
 Homo sapiens
3.1.4.12 alk-SMase
-
 Rattus norvegicus
3.1.4.12 alkaline sphingomyelinase
-
 Homo sapiens
3.1.4.12 alkaline sphingomyelinase
-
 Rattus norvegicus
3.1.4.12 intestinal alkaline sphingomyelinase
-
 Homo sapiens
3.1.4.12 intestinal alkaline sphingomyelinase
-
 Rattus norvegicus
3.1.4.12 More alk-SMase is a member of the NPP, i.e. nucleotide diphosphatase/phosphodiesterase, family Homo sapiens
3.1.4.12 More alk-SMase is a member of the NPP, i.e. nucleotide diphosphatase/phosphodiesterase, family Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.4.12 37
-
 assay at Homo sapiens
3.1.4.12 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.4.12 7.5
-
 hydrolysis of platelet-activating factor Rattus norvegicus
3.1.4.12 7.5
-
 alk-SMase activity with platelet-inactivating factor Homo sapiens
3.1.4.12 7.5
-
 alk-SMase activity with platelet-inactivating factor Rattus norvegicus
3.1.4.12 9
-
 hydrolysis of sphingomyelin Rattus norvegicus
3.1.4.12 9
-
 alk-SMase activity on sphingomyelin Homo sapiens
3.1.4.12 9
-
 alk-SMase activity on sphingomyelin Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.4.12 5 10
-
 Homo sapiens
3.1.4.12 5 10
-
 Rattus norvegicus
3.1.4.12 6 9 pH 6.0: about 55% of maximal activity, pH 9.0: about 35% of maximal activity, hydrolysis of platelet-activating factor Rattus norvegicus