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Literature summary extracted from

  • Kim, S.; Lee, S.B.
    Identification and characterization of Sulfolobus solfataricus D-gluconate dehydratase: a key enzyme in the non-phosphorylated Entner-Doudoroff pathway (2005), Biochem. J., 387, 271-280.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.1.39 2-mercaptoethanol 1 mM, 17% inhibition Saccharolobus solfataricus
4.2.1.39 dithiothreitol 1 mM, 24% inhibition Saccharolobus solfataricus
4.2.1.39 EDTA
-
 Saccharolobus solfataricus
4.2.1.39 glutathione 1 mM, 41% inhibition Saccharolobus solfataricus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.1.39 0.45
-
 D-gluconate
-
 Saccharolobus solfataricus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.1.39 Co2+ 1 mM, activates. Presence of one Co2+ per enzyme monomer Saccharolobus solfataricus
4.2.1.39 Mg2+ 1 mM, activates Saccharolobus solfataricus
4.2.1.39 Mn2+ 1 mM, activates Saccharolobus solfataricus
4.2.1.39 Ni2+ 1 mM, activates Saccharolobus solfataricus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.1.39 44000
-
 8 * 44000, SDS-PAGE Saccharolobus solfataricus
4.2.1.39 350000
-
 gel filtration Saccharolobus solfataricus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.1.39 D-gluconate Saccharolobus solfataricus key enzyme in the non-phosphorylated Entner-Doudoroff pathway 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.1.39 Saccharolobus solfataricus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
4.2.1.39 phosphoprotein phosphorylation and dephosphorylation control the activity of D-gluconate dehydratase, loss of activity by dephosphorylation Saccharolobus solfataricus

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.1.39
-
 Saccharolobus solfataricus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.1.39 26.04
-
-
 Saccharolobus solfataricus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.1.39 D-galactoheptonate 1.6% of the activity with D-gluconate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactoheptonate + H2O
-
 ?
4.2.1.39 D-galactonate 2.8% of the activity with D-gluconate. Studies by other groups reveal a catalytic efficiency of 20% for D-galactonate compared to D-gluconate Saccharolobus solfataricus 2-dehydro-3-deoxy-D-galactonate + H2O
-
 ?
4.2.1.39 D-gluconate
-
 Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?
4.2.1.39 D-gluconate key enzyme in the non-phosphorylated Entner-Doudoroff pathway Saccharolobus solfataricus 2-dehydro-3-deoxy-D-gluconate + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.1.39 octamer 8 * 44000, SDS-PAGE Saccharolobus solfataricus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.1.39 80 90
-
 Saccharolobus solfataricus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
4.2.1.39 70 95 70°C: about 40% of maximal activity, 95°C: about 40% of maximal activity Saccharolobus solfataricus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.1.39 80
-
 stable for 2 h Saccharolobus solfataricus
4.2.1.39 90
-
 half-life: 120 min Saccharolobus solfataricus
4.2.1.39 100
-
 half-life: less than 40 min Saccharolobus solfataricus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.1.39 760
-
 D-gluconate
-
 Saccharolobus solfataricus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.1.39 6.5 7.5
-
 Saccharolobus solfataricus