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Literature summary extracted from

  • Kipper, K.; Vaeljamaee, P.; Johansson, G.
    Processive action of cellobiohydrolase Cel7A from Trichoderma reesei is revealed as burst kinetics on fluorescent polymeric model substrates (2005), Biochem. J., 385, 527-535.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 additional information
-
 additional information active-site titration kinetics of Cel7A, i.e. burst kinetics, for quantitation of the progressivity of the enzyme, overview Trichoderma reesei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.91 extracellular secereted enzymes Trichoderma reesei
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.91 cellulose + H2O Trichoderma reesei
-
 cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O Trichoderma reesei QM9414
-
 cellobiose + ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Trichoderma reesei
-
-
-
3.2.1.91 Trichoderma reesei QM9414
-
-
-
3.2.1.176 Trichoderma reesei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.91 extracellular Cel5A, Cel6A, and Cel7A from culture medium Trichoderma reesei

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.91 2 cellohexaose + 2 H2O = 2 cellotriose + cellotetraose + cellobiose progressivity and reaction mechanism of Cel7A Trichoderma reesei
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.176 additional information
-
 the processive action of reducing-end-specific Trichoderma reesei cellobiohydrolase Cel7A on derivatized cellulose model substrates allows to perform an active-site titration, which in turn can be used to quantify the processivity of the enzyme Trichoderma reesei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma reesei 4-nitrophenol + beta-D-cellobiose
-
 ?
3.2.1.91 4-nitrophenyl beta-D-cellobioside + H2O
-
 Trichoderma reesei QM9414 4-nitrophenol + beta-D-cellobiose
-
 ?
3.2.1.91 cellulose + H2O
-
 Trichoderma reesei cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O the enzyme acts on anthranilic-acid-labelled bacterial cellulose, bacterial microcrystalline cellulose, and endoglucanase-pretreated bacterial cellulose, preparation, overview Trichoderma reesei cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O
-
 Trichoderma reesei QM9414 cellobiose + ?
-
 ?
3.2.1.91 cellulose + H2O the enzyme acts on anthranilic-acid-labelled bacterial cellulose, bacterial microcrystalline cellulose, and endoglucanase-pretreated bacterial cellulose, preparation, overview Trichoderma reesei QM9414 cellobiose + ?
-
 ?
3.2.1.91 additional information Cel5A treated cellulose is no longer a substrate for Cel6A or Cel7A Trichoderma reesei ?
-
 ?
3.2.1.91 additional information Cel5A treated cellulose is no longer a substrate for Cel6A or Cel7A Trichoderma reesei QM9414 ?
-
 ?
3.2.1.176 cellulose + H2O Cel7A acts on labelled bacterial cellulose, bacterial microcrystalline cellulose and endoglucanase-pretreated bacterial cellulose respectively Trichoderma reesei cellobiose + ?
-
 ?
3.2.1.176 cellulose + H2O Cel7A acts on labelled bacterial cellulose, bacterial microcrystalline cellulose and endoglucanase-pretreated bacterial cellulose respectively Trichoderma reesei QM9414 cellobiose + ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.91 Cel5A
-
 Trichoderma reesei
3.2.1.91 Cel6A
-
 Trichoderma reesei
3.2.1.91 Cel7A formerly CBH I Trichoderma reesei
3.2.1.91 cellobiohydrolase
-
 Trichoderma reesei
3.2.1.176 Cel7A
-
 Trichoderma reesei

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.91 25
-
 assay at Trichoderma reesei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.91 5
-
 assay at Trichoderma reesei