BRENDA - Enzyme Database

Cryptococcal phospholipases: a novel lysophospholipase discovered in the pathogenic fungus Cryptococcus gattii

Wright, L.C.; Payne, J.; Santangelo, R.T.; Simpanya, M.F.; Chen, S.C.; Widmer, F.; Sorrell, T.C.; Biochem. J. 384, 377-384 (2004)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.5
carnitine
10 mM, activation to 121% of control at pH 5.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
dithiothreitol
10 mM, activation to 122% of control at pH 5.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
EGTA
10 mM, 11% activation of phospholipase B activity
Cryptococcus gattii VGIII
3.1.1.5
lysophosphatidylcholine
0.5 mM, activation of phospholipase B activity to 132% of control
Cryptococcus gattii VGIII
3.1.1.5
palmitoyl carnitine
0.5 mM, stimulates to 157% of control at pH 7.0, LPL1; 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control
Cryptococcus gattii VGIII
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
CaCl2
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity
Cryptococcus gattii VGIII
3.1.1.5
carnitine
10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
dipalmitoyl phosphatidylcholine
0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1
Cryptococcus gattii VGIII
3.1.1.5
dipalmitoylphosphatidylcholine
0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
dithiothreitol
10 mM, 9% inhibition at pH 7.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
EGTA
10 mM, 15% inhibition at pH 7.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
FeCl3
10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
palmitoyl carnitine
0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0
Cryptococcus gattii VGIII
3.1.1.5
phosphatidic acid
0.5 mM, 24% inhibition at pH 5.0, 66% inhibition at pH 7.0, LPL1; 0.5 mM, 58% inhibition of phospholipase B activity, 92% inhibition of lysophospholipase at pH 5.0, 87% inhibition of lysophospholipase at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
sphingomyelin
0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
Triton X-100
0.1 w/v, 48% inhibition at pH 5.0, LPL1; 0.1% w/v, 85% inhibition of phospholipase B activity, 91% inhibition of lysophospholipase B activity at pH 5.0, 95% inhibition of lysophospholipase B activity at pH 7.0
Cryptococcus gattii VGIII
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.5
Ca2+
10 mM, stimulates to 260% of control at pH 7.0, but not at pH 5.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
CaCl2
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0
Cryptococcus gattii VGIII
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
66000
-
10 * 66000, isoenzyme LPL1, SDS-PAGE
Cryptococcus gattii VGIII
3.1.1.5
220000
-
isoenzyme LPL1, gel filtratiin
Cryptococcus neoformans
3.1.1.5
275000
-
isoenzyme PLB1, gel filtratiin
Cryptococcus gattii VGIII
3.1.1.5
670000
-
-
Cryptococcus gattii VGIII
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.1.1.5
Cryptococcus gattii VGIII
-
-
-
3.1.1.5
Cryptococcus neoformans
-
var. grubii, strain H99
-
Posttranslational Modification
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
glycoprotein
-
Cryptococcus gattii VGIII
Purification (Commentary)
EC Number
Commentary
Organism
3.1.1.5
isoenzyme LPL1
Cryptococcus neoformans
3.1.1.5
isoenzyme PLB1
Cryptococcus gattii VGIII
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
1-oleoyl lysophosphatidylcholine + H2O
30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
663943
Cryptococcus gattii VGIII
glycerophosphocholine + oleate
-
-
-
?
3.1.1.5
1-oleoyl-2-lysophosphatidylcholine + H2O
30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0
663943
Cryptococcus gattii VGIII
glycerophosphorylcholine + oleic acid
-
-
-
?
3.1.1.5
1-palmitoyl lysophosphatidylcholine + H2O
preferred substrate, LPL1
663943
Cryptococcus gattii VGIII
glycerophosphocholine + palmitate
-
-
-
?
3.1.1.5
1-palmitoyl lysophosphatidylcholine + H2O
-
663943
Cryptococcus gattii VGIII
glycerophosphocholine + palmitic acid
-
-
-
?
3.1.1.5
1-palmitoyl-lysophosphatidylethanolamine + H2O
as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0
663943
Cryptococcus gattii VGIII
glycerophosphatidylethanolamine + palmitic acid
-
-
-
?
3.1.1.5
1-palmitoyl-lysophosphatidylethanolamine + H2O
20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
663943
Cryptococcus gattii VGIII
glycerophosphoethanolamine + palmitate
-
-
-
?
3.1.1.5
1-palmitoyl-sn-glycero-3-phosphoserine + H2O
as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0, 70% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0
663943
Cryptococcus gattii VGIII
glycerophosphoserine + palmitic acid
-
-
-
?
3.1.1.5
dioleoylphosphatidylcholine + H2O
2% of the activity with dipalmitoylphosphatidylcholine
663943
Cryptococcus gattii VGIII
?
-
-
-
?
3.1.1.5
dioleoylphosphatidylethanolamine + H2O
11% of the activity with dipalmitoylphosphatidylcholine
663943
Cryptococcus gattii VGIII
?
-
-
-
?
3.1.1.5
dipalmitoylphosphatidylcholine + H2O
-
663943
Cryptococcus gattii VGIII
?
-
-
-
?
3.1.1.5
additional information
PLB1 shows phospholipase B activity, lysophospholipase activity and transacylase activity
663943
Cryptococcus gattii VGIII
?
-
-
-
-
3.1.1.5
additional information
the LPL1 protein only possesses lysophospholipase and transacylase activity
663943
Cryptococcus neoformans
?
-
-
-
-
3.1.1.5
additional information
the LPL1 protein only possesses lysophospholipase and transacylase activity
663943
Cryptococcus gattii VGIII
?
-
-
-
-
3.1.1.5
sphingomyelin + H2O
3% of the activity with dipalmitoylphosphatidylcholine
663943
Cryptococcus gattii VGIII
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.1.1.5
decamer
10 * 66000, isoenzyme LPL1, SDS-PAGE
Cryptococcus gattii VGIII
Temperature Optimum [C]
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.1.5
37
-
; phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1, no activity detectable at higher temperatures
Cryptococcus gattii VGIII
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
4.5
5
phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1
Cryptococcus gattii VGIII
3.1.1.5
5
-
lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1
Cryptococcus gattii VGIII
3.1.1.5
5.5
8
lysophospholipase activity, long incubation time (5 min), LPL1
Cryptococcus gattii VGIII
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.5
4.2
6
pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1
Cryptococcus gattii VGIII
3.1.1.5
7
-
no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min
Cryptococcus gattii VGIII
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.1.5
Cryptococcus gattii VGIII
isoelectric focusing
-
3.9
3.1.1.5
Cryptococcus gattii VGIII
isoelectric focusing, isoenzyme LPL1
-
6.3
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.1.1.5
carnitine
10 mM, activation to 121% of control at pH 5.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
dithiothreitol
10 mM, activation to 122% of control at pH 5.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
EGTA
10 mM, 11% activation of phospholipase B activity
Cryptococcus gattii VGIII
3.1.1.5
lysophosphatidylcholine
0.5 mM, activation of phospholipase B activity to 132% of control
Cryptococcus gattii VGIII
3.1.1.5
palmitoyl carnitine
0.5 mM, stimulates to 157% of control at pH 7.0, LPL1; 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control
Cryptococcus gattii VGIII
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.1.1.5
CaCl2
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity
Cryptococcus gattii VGIII
3.1.1.5
carnitine
10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
dipalmitoyl phosphatidylcholine
0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1
Cryptococcus gattii VGIII
3.1.1.5
dipalmitoylphosphatidylcholine
0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
dithiothreitol
10 mM, 9% inhibition at pH 7.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
EGTA
10 mM, 15% inhibition at pH 7.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
FeCl3
10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
palmitoyl carnitine
0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0
Cryptococcus gattii VGIII
3.1.1.5
phosphatidic acid
0.5 mM, 24% inhibition at pH 5.0, 66% inhibition at pH 7.0, LPL1; 0.5 mM, 58% inhibition of phospholipase B activity, 92% inhibition of lysophospholipase at pH 5.0, 87% inhibition of lysophospholipase at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
sphingomyelin
0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0
Cryptococcus gattii VGIII
3.1.1.5
Triton X-100
0.1 w/v, 48% inhibition at pH 5.0, LPL1; 0.1% w/v, 85% inhibition of phospholipase B activity, 91% inhibition of lysophospholipase B activity at pH 5.0, 95% inhibition of lysophospholipase B activity at pH 7.0
Cryptococcus gattii VGIII
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.1.1.5
Ca2+
10 mM, stimulates to 260% of control at pH 7.0, but not at pH 5.0, LPL1
Cryptococcus gattii VGIII
3.1.1.5
CaCl2
10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0
Cryptococcus gattii VGIII
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.1.1.5
66000
-
10 * 66000, isoenzyme LPL1, SDS-PAGE
Cryptococcus gattii VGIII
3.1.1.5
220000
-
isoenzyme LPL1, gel filtratiin
Cryptococcus neoformans
3.1.1.5
275000
-
isoenzyme PLB1, gel filtratiin
Cryptococcus gattii VGIII
3.1.1.5
670000
-
-
Cryptococcus gattii VGIII
Posttranslational Modification (protein specific)
EC Number
Posttranslational Modification
Commentary
Organism
3.1.1.5
glycoprotein
-
Cryptococcus gattii VGIII
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.1.1.5
isoenzyme LPL1
Cryptococcus neoformans
3.1.1.5
isoenzyme PLB1
Cryptococcus gattii VGIII
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.1.1.5
1-oleoyl lysophosphatidylcholine + H2O
30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
663943
Cryptococcus gattii VGIII
glycerophosphocholine + oleate
-
-
-
?
3.1.1.5
1-oleoyl-2-lysophosphatidylcholine + H2O
30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0
663943
Cryptococcus gattii VGIII
glycerophosphorylcholine + oleic acid
-
-
-
?
3.1.1.5
1-palmitoyl lysophosphatidylcholine + H2O
preferred substrate, LPL1
663943
Cryptococcus gattii VGIII
glycerophosphocholine + palmitate
-
-
-
?
3.1.1.5
1-palmitoyl lysophosphatidylcholine + H2O
-
663943
Cryptococcus gattii VGIII
glycerophosphocholine + palmitic acid
-
-
-
?
3.1.1.5
1-palmitoyl-lysophosphatidylethanolamine + H2O
as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0
663943
Cryptococcus gattii VGIII
glycerophosphatidylethanolamine + palmitic acid
-
-
-
?
3.1.1.5
1-palmitoyl-lysophosphatidylethanolamine + H2O
20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1
663943
Cryptococcus gattii VGIII
glycerophosphoethanolamine + palmitate
-
-
-
?
3.1.1.5
1-palmitoyl-sn-glycero-3-phosphoserine + H2O
as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0, 70% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0
663943
Cryptococcus gattii VGIII
glycerophosphoserine + palmitic acid
-
-
-
?
3.1.1.5
dioleoylphosphatidylcholine + H2O
2% of the activity with dipalmitoylphosphatidylcholine
663943
Cryptococcus gattii VGIII
?
-
-
-
?
3.1.1.5
dioleoylphosphatidylethanolamine + H2O
11% of the activity with dipalmitoylphosphatidylcholine
663943
Cryptococcus gattii VGIII
?
-
-
-
?
3.1.1.5
dipalmitoylphosphatidylcholine + H2O
-
663943
Cryptococcus gattii VGIII
?
-
-
-
?
3.1.1.5
additional information
PLB1 shows phospholipase B activity, lysophospholipase activity and transacylase activity
663943
Cryptococcus gattii VGIII
?
-
-
-
-
3.1.1.5
additional information
the LPL1 protein only possesses lysophospholipase and transacylase activity
663943
Cryptococcus neoformans
?
-
-
-
-
3.1.1.5
additional information
the LPL1 protein only possesses lysophospholipase and transacylase activity
663943
Cryptococcus gattii VGIII
?
-
-
-
-
3.1.1.5
sphingomyelin + H2O
3% of the activity with dipalmitoylphosphatidylcholine
663943
Cryptococcus gattii VGIII
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.1.1.5
decamer
10 * 66000, isoenzyme LPL1, SDS-PAGE
Cryptococcus gattii VGIII
Temperature Optimum [C] (protein specific)
EC Number
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
3.1.1.5
37
-
; phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1, no activity detectable at higher temperatures
Cryptococcus gattii VGIII
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.1.1.5
4.5
5
phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1
Cryptococcus gattii VGIII
3.1.1.5
5
-
lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1
Cryptococcus gattii VGIII
3.1.1.5
5.5
8
lysophospholipase activity, long incubation time (5 min), LPL1
Cryptococcus gattii VGIII
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.1.1.5
4.2
6
pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1
Cryptococcus gattii VGIII
3.1.1.5
7
-
no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min
Cryptococcus gattii VGIII
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.1.1.5
Cryptococcus gattii VGIII
isoelectric focusing
-
3.9
3.1.1.5
Cryptococcus gattii VGIII
isoelectric focusing, isoenzyme LPL1
-
6.3