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Literature summary extracted from

  • Wright, L.C.; Payne, J.; Santangelo, R.T.; Simpanya, M.F.; Chen, S.C.; Widmer, F.; Sorrell, T.C.
    Cryptococcal phospholipases: a novel lysophospholipase discovered in the pathogenic fungus Cryptococcus gattii (2004), Biochem. J., 384, 377-384.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.5 carnitine 10 mM, activation to 121% of control at pH 5.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 dithiothreitol 10 mM, activation to 122% of control at pH 5.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 EGTA 10 mM, 11% activation of phospholipase B activity Cryptococcus gattii VGIII
3.1.1.5 lysophosphatidylcholine 0.5 mM, activation of phospholipase B activity to 132% of control Cryptococcus gattii VGIII
3.1.1.5 palmitoyl carnitine 0.5 mM, stimulates to 157% of control at pH 7.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 palmitoyl carnitine 1 mM, activation of lysophospholipase activity at pH 7.0 to 239% of control Cryptococcus gattii VGIII

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.5 CaCl2 10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity Cryptococcus gattii VGIII
3.1.1.5 carnitine 10 mM, 38% inhibition of phospholipase B activity; 10 mM, 9% inhibition at pH 7.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 dipalmitoyl phosphatidylcholine 0.5 mM, 84% inhibition at pH 5.0, 50% inhibition at pH 7.0, LPL 1 Cryptococcus gattii VGIII
3.1.1.5 dipalmitoylphosphatidylcholine 0.5 mM, 89% inhibition of lysophospholipase at pH 5.0, 82% inhibition of lysophospholipase at pH 7.0 Cryptococcus gattii VGIII
3.1.1.5 dithiothreitol 10 mM, 9% inhibition at pH 7.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 EGTA 10 mM, 15% inhibition at pH 7.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 FeCl3 10 mM, 39% inhibition at pH 5.0, 38% inhibition at pH 7.0, LPL1; 10 mM, 80% inhibiotion of phospholipase B activity, 17% inhibition of lysophospholipase activity at pH 5.0, 29% in inhibition of lysophospholipase activity at pH 7.0 Cryptococcus gattii VGIII
3.1.1.5 palmitoyl carnitine 0.5 mM, 90% inhibition at pH 5.0, LPL1; 1 mM, 64% inhibition of phospholipase B activity, 65% inhibition of lysophospholipase activity at pH 5.0 Cryptococcus gattii VGIII
3.1.1.5 phosphatidic acid 0.5 mM, 24% inhibition at pH 5.0, 66% inhibition at pH 7.0, LPL1; 0.5 mM, 58% inhibition of phospholipase B activity, 92% inhibition of lysophospholipase at pH 5.0, 87% inhibition of lysophospholipase at pH 7.0 Cryptococcus gattii VGIII
3.1.1.5 sphingomyelin 0.5 MM, 21% inhibition at pH 5.0, 29% inhibition at pH 7.0, LPL1; 0.5 mM, 35% inhibition of phospholipase B activity, 45% inhibition of lysophospholipase at pH 5.0, 28% inhibition of lysophospholipase at pH 7.0 Cryptococcus gattii VGIII
3.1.1.5 Triton X-100 0.1 w/v, 48% inhibition at pH 5.0, LPL1; 0.1% w/v, 85% inhibition of phospholipase B activity, 91% inhibition of lysophospholipase B activity at pH 5.0, 95% inhibition of lysophospholipase B activity at pH 7.0 Cryptococcus gattii VGIII

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.1.1.5 Ca2+ 10 mM, stimulates to 260% of control at pH 7.0, but not at pH 5.0, LPL1 Cryptococcus gattii VGIII
3.1.1.5 CaCl2 10 mM, 22% inhibition of phospholipase B activity, slight activation of lysophospholipase activity, to 8% at pH 5.0 and 4% at pH 7.0 Cryptococcus gattii VGIII

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.5 66000
-
 10 * 66000, isoenzyme LPL1, SDS-PAGE Cryptococcus gattii VGIII
3.1.1.5 220000
-
 isoenzyme LPL1, gel filtratiin Cryptococcus neoformans
3.1.1.5 275000
-
 isoenzyme PLB1, gel filtratiin Cryptococcus gattii VGIII
3.1.1.5 670000
-
-
 Cryptococcus gattii VGIII

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.5 Cryptococcus gattii VGIII
-
-
-
3.1.1.5 Cryptococcus neoformans
-
 var. grubii, strain H99
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.5 glycoprotein
-
 Cryptococcus gattii VGIII

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.5 isoenzyme LPL1 Cryptococcus neoformans
3.1.1.5 isoenzyme PLB1 Cryptococcus gattii VGIII

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.5 1-oleoyl lysophosphatidylcholine + H2O 30% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1 Cryptococcus gattii VGIII glycerophosphocholine + oleate
-
 ?
3.1.1.5 1-oleoyl-2-lysophosphatidylcholine + H2O 30% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0, 667% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 7.0 Cryptococcus gattii VGIII glycerophosphorylcholine + oleic acid
-
 ?
3.1.1.5 1-palmitoyl lysophosphatidylcholine + H2O preferred substrate, LPL1 Cryptococcus gattii VGIII glycerophosphocholine + palmitate
-
 ?
3.1.1.5 1-palmitoyl lysophosphatidylcholine + H2O
-
 Cryptococcus gattii VGIII glycerophosphocholine + palmitic acid
-
 ?
3.1.1.5 1-palmitoyl-lysophosphatidylethanolamine + H2O as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0 Cryptococcus gattii VGIII glycerophosphatidylethanolamine + palmitic acid
-
 ?
3.1.1.5 1-palmitoyl-lysophosphatidylethanolamine + H2O 20% of the activity with 1-palmitoyl lysophosphatidylcholine, LPL1 Cryptococcus gattii VGIII glycerophosphoethanolamine + palmitate
-
 ?
3.1.1.5 1-palmitoyl-sn-glycero-3-phosphoserine + H2O as active as 1-palmitoyl lysophosphatidylcholine at pH 7.0, 70% of the activity with 1-palmitoyl lysophosphatidylcholine at pH 5.0 Cryptococcus gattii VGIII glycerophosphoserine + palmitic acid
-
 ?
3.1.1.5 dioleoylphosphatidylcholine + H2O 2% of the activity with dipalmitoylphosphatidylcholine Cryptococcus gattii VGIII ?
-
 ?
3.1.1.5 dioleoylphosphatidylethanolamine + H2O 11% of the activity with dipalmitoylphosphatidylcholine Cryptococcus gattii VGIII ?
-
 ?
3.1.1.5 dipalmitoylphosphatidylcholine + H2O
-
 Cryptococcus gattii VGIII ?
-
 ?
3.1.1.5 additional information PLB1 shows phospholipase B activity, lysophospholipase activity and transacylase activity Cryptococcus gattii VGIII ?
-
 ?
3.1.1.5 additional information the LPL1 protein only possesses lysophospholipase and transacylase activity Cryptococcus neoformans ?
-
 ?
3.1.1.5 additional information the LPL1 protein only possesses lysophospholipase and transacylase activity Cryptococcus gattii VGIII ?
-
 ?
3.1.1.5 sphingomyelin + H2O 3% of the activity with dipalmitoylphosphatidylcholine Cryptococcus gattii VGIII ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.5 decamer 10 * 66000, isoenzyme LPL1, SDS-PAGE Cryptococcus gattii VGIII

Synonyms

EC Number Synonyms Comment Organism
3.1.1.5 LPL1
-
 Cryptococcus neoformans
3.1.1.5 LPL1
-
 Cryptococcus gattii VGIII
3.1.1.5 PLB1 enzyme
-
 Cryptococcus gattii VGIII

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.5 37
-
-
 Cryptococcus gattii VGIII
3.1.1.5 37
-
 phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1, no activity detectable at higher temperatures Cryptococcus gattii VGIII

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.5 4.5 5 phospholipase B activity, lysophospholipase activity and transacylase activity of PLB1 Cryptococcus gattii VGIII
3.1.1.5 5
-
 lysophospholipase activity and transacylase activity, short incubation time (30 s), LPL1 Cryptococcus gattii VGIII
3.1.1.5 5.5 8 lysophospholipase activity, long incubation time (5 min), LPL1 Cryptococcus gattii VGIII

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.5 4.2 6 pH 4.2: about 75% of maximal lysophospholipase activity, pH 6.0: about 75% of maximal lysophospholipase activity, short incubation time (30 s), LPL1 Cryptococcus gattii VGIII
3.1.1.5 7
-
 no phospholipase B activity, no lysophospholipase activity and no transacylase activity of PLB1 is detected at short incubation time (30 s), at incubation time 5-10 min lysophospholipase activity and transacylase activity is detected (about 5% of the activity at pH 5.0), no phospholipase B activity is detected at incubation time 5-10 min Cryptococcus gattii VGIII

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.5 Cryptococcus gattii VGIII isoelectric focusing
-
 3.9
3.1.1.5 Cryptococcus gattii VGIII isoelectric focusing, isoenzyme LPL1
-
 6.3