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Literature summary extracted from

  • Abbadi, A.; Brummel, M.; Schutt, B.S.; Slabaugh, M.B.; Schuch, R.; Spener, F.
    Reaction mechanism of recombinant 3-oxoacyl-(acyl-carrier-protein) synthase III from Cuphea wrightii embryo, a fatty acid synthase type II condensing enzyme (2000), Biochem. J., 345, 153-160.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.180 expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Cuphea wrightii

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.180 C111A site-directed mutagenesis, the mutant enzyme loses all condensing activity Cuphea wrightii
2.3.1.180 C111S site-directed mutagenesis, the mutant enzyme loses all condensing activity, but is still able to bind acetyl-CoA and malonyl-ACP and to decarboxylate the latter to acetyl-ACP Cuphea wrightii
2.3.1.180 C111S/H261A site-directed mutagenesis, the mutant enzyme loses all condensing activity Cuphea wrightii
2.3.1.180 C111S/H261R site-directed mutagenesis, the mutant enzyme loses all condensing activity, but is still able to bind acetyl-CoA and malonyl-ACP and to decarboxylate the latter to acetyl-ACP Cuphea wrightii
2.3.1.180 H261A site-directed mutagenesis, the mutant enzyme loses all condensing activity Cuphea wrightii
2.3.1.180 H261R site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme Cuphea wrightii
2.3.1.180 R150A site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme Cuphea wrightii
2.3.1.180 R266A site-directed mutagenesis, the mutant enzyme shows reduced condensing activity compared to the wild-type enzyme Cuphea wrightii
2.3.1.180 R306A site-directed mutagenesis, the mutant enzyme shows highly reduced condensing activity compared to the wild-type enzyme Cuphea wrightii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.180 additional information
-
 additional information kinetics Cuphea wrightii
2.3.1.180 0.0014
-
 acetyl-CoA pH 7.6, 30°C, recombinant His-tagged wild-type enzyme Cuphea wrightii
2.3.1.180 0.0041
-
 malonyl-ACP pH 7.6, 30°C, recombinant His-tagged wild-type enzyme Cuphea wrightii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein] Cuphea wrightii
-
 acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.180 Cuphea wrightii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.180 recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) Cuphea wrightii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.180 acetyl-CoA + a malonyl-[acyl-carrier protein] = an acetoacetyl-[acyl-carrier protein] + CoA + CO2 ping pong reaction mechanism, catalysis involves His261, Arg150, and Arg306, determination of substrate binding site Cuphea wrightii
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.180 embryo
-
 Cuphea wrightii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.1.180 0.2
-
 purified recombinant His-tagged mutant R306A Cuphea wrightii
2.3.1.180 0.8
-
 purified recombinant His-tagged mutant H261R Cuphea wrightii
2.3.1.180 1.9
-
 purified recombinant His-tagged mutant R150A Cuphea wrightii
2.3.1.180 3.5
-
 purified recombinant His-tagged mutant R266A Cuphea wrightii
2.3.1.180 7
-
 purified recombinant His-tagged wild-type enzyme Cuphea wrightii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.180 acetyl-CoA + malonyl-[acyl-carrier protein]
-
 Cuphea wrightii acetoacetyl-[acyl-carrier protein] + CoA + CO2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.180 3-oxoacyl-[acyl-carrier-protein] synthase III
-
 Cuphea wrightii
2.3.1.180 ACP synthase III
-
 Cuphea wrightii
2.3.1.180 fatty acid synthase type II condensing enzyme
-
 Cuphea wrightii
2.3.1.180 KAS III
-
 Cuphea wrightii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.180 30
-
 assay at Cuphea wrightii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.180 7.6
-
 assay at Cuphea wrightii