EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.5.1.2 | by addition of ammonium sulfate | Pseudomonas aeruginosa |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.5.1.2 | HgCl2 | - |
Pseudomonas aeruginosa | |
3.5.1.2 | additional information | no inhibition by HgCl2; no inhibition by p-chloromercuribenzoate | Pseudomonas aeruginosa | |
3.5.1.2 | p-chloromercuribenzoate | - |
Pseudomonas aeruginosa |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.5.1.2 | 0.11 | - |
L-glutamine | - |
Pseudomonas aeruginosa | |
3.5.1.2 | 0.18 | - |
L-glutamine | - |
Pseudomonas aeruginosa |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.5.1.2 | 67000 | - |
gel filtration | Pseudomonas aeruginosa |
3.5.1.2 | 137000 | - |
sedimentation equilibrium mehod | Pseudomonas aeruginosa |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.2 | L-glutamine + H2O | Pseudomonas aeruginosa | - |
L-glutamate + NH3 | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.5.1.2 | Pseudomonas aeruginosa | - |
IFO 3080 | - |
3.5.1.2 | Pseudomonas aeruginosa | Q9I387 | IFO 3080 | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.5.1.2 | - |
Pseudomonas aeruginosa |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.5.1.2 | 22.1 | - |
purified enzyme | Pseudomonas aeruginosa |
3.5.1.2 | 27.1 | - |
purified enzyme | Pseudomonas aeruginosa |
EC Number | Storage Stability | Organism |
---|---|---|
3.5.1.2 | 0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little lose of activity | Pseudomonas aeruginosa |
3.5.1.2 | 0-5°C, 0.01 M potassium phosphate buffer, pH 7.2, containing 12.5% glycerol, little loss of activity | Pseudomonas aeruginosa |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.5.1.2 | D-asparagine + H2O | 44% activity | Pseudomonas aeruginosa | D-aspartate + NH3 | - |
? | |
3.5.1.2 | D-glutamine + H2O | 65% activity | Pseudomonas aeruginosa | D-glutamate + NH3 | - |
? | |
3.5.1.2 | D-glutamine + H2O | 92% activity | Pseudomonas aeruginosa | D-glutamate + NH3 | - |
? | |
3.5.1.2 | D-theanine + H2O | 57% activity | Pseudomonas aeruginosa | D-glutamate + ethylamine | - |
? | |
3.5.1.2 | L-asparagine + H2O | 51% activity | Pseudomonas aeruginosa | L-aspartate + NH3 | - |
? | |
3.5.1.2 | L-gamma-glutamyl-p-nitroanilide + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
3.5.1.2 | L-gamma-glutamylhydrazine + H2O | - |
Pseudomonas aeruginosa | ? | - |
? | |
3.5.1.2 | L-glutamine + H2O | - |
Pseudomonas aeruginosa | L-glutamate + NH3 | - |
? | |
3.5.1.2 | L-glutamine + H2O | pH 7.2, 30°C | Pseudomonas aeruginosa | L-glutamate + NH3 | - |
? | |
3.5.1.2 | L-theanine + H2O | 71% activity | Pseudomonas aeruginosa | L-glutamate + ethylamine | - |
? | |
3.5.1.2 | additional information | no reaction with L-theanine, D-theanine, L-gamma-glutamylhydrazine, and L-gamma-glutamyl-p-nitroanilide | Pseudomonas aeruginosa | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.5.1.2 | glutaminase A | - |
Pseudomonas aeruginosa |
3.5.1.2 | glutaminase B | - |
Pseudomonas aeruginosa |
3.5.1.2 | L-glutamine amidohydrolase | - |
Pseudomonas aeruginosa |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.2 | 8.5 | - |
- |
Pseudomonas aeruginosa |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.5.1.2 | 7.5 | 9 | - |
Pseudomonas aeruginosa |