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Literature summary extracted from

  • Nakayama-Hamada, M.; Suzuki, A.; Kubota, K.; Takazawa, T.; Ohsaka, M.; Kawaida, R.; Ono, M.; Kasuya, A.; Furukawa, H.; Yamada, R.; Yamamoto, K.
    Comparison of enzymatic properties between hPADI2 and hPADI4 (2005), Biochem. Biophys. Res. Commun., 327, 192-200.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.3.15 hPADI2 is expressed in Escherichia coli Homo sapiens
3.5.3.15 hPADI4 is expressed in Escherichia coli Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.3.15 0.001
-
 filaggrin hPADI2 Homo sapiens
3.5.3.15 0.013
-
 fibrinogen hPADI2 Homo sapiens
3.5.3.15 0.055
-
 fibrinogen hPADI4 Homo sapiens
3.5.3.15 0.064
-
 filaggrin hPADI4 Homo sapiens
3.5.3.15 0.15
-
 benzoyl-Arg-ethyl ester pH 7.6, 37°C, hPADI2 expressed by baculovirus Homo sapiens
3.5.3.15 0.16
-
 benzoyl-Arg-NH2 pH 7.6, 37°C, hPADI4 expressed in Escherichia coli Homo sapiens
3.5.3.15 0.63
-
 benzoyl-Arg-methyl ester pH 7.6, 37°C, hPADI4 expressed in Escherichia coli Homo sapiens
3.5.3.15 0.73
-
 benzoyl-Arg-ethyl ester pH 7.6, 37°C, hPADI2 expressed in Escherichia coli Homo sapiens
3.5.3.15 0.77
-
 benzoyl-Arg-methyl ester pH 7.6, 37°C, hPADI2 expressed by baculovirus Homo sapiens
3.5.3.15 0.91
-
 benzoyl-Arg-ethyl ester pH 7.6, 37°C, hPADI4 expressed in Escherichia coli Homo sapiens
3.5.3.15 1.01
-
 benzoyl-Arg-NH2 pH 7.6, 37°C, hPADI2 expressed in Escherichia coli Homo sapiens
3.5.3.15 1.23
-
 benzoyl-Arg-NH2 pH 7.6, 37°C, hPADI2 expressed by baculovirus Homo sapiens
3.5.3.15 1.63
-
 tosyl-Arg-ethyl ester pH 7.6, 37°C, hPADI2 expressed by baculovirus Homo sapiens
3.5.3.15 1.97
-
 benzoyl-Arg-methyl ester pH 7.6, 37°C, hPADI2 expressed in Escherichia coli Homo sapiens
3.5.3.15 5.61
-
 tosyl-Arg-ethyl ester pH 7.6, 37°C, hPADI2 expressed in Escherichia coli Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.3.15 cytoplasm hPADI2 Homo sapiens 5737
-
3.5.3.15 cytoplasm hPADI4 Homo sapiens 5737
-
3.5.3.15 nucleus hPADI4 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.3.15 Ca2+ increase activity of hPADI4. 50% of maximal activity at 0.75 mM Homo sapiens
3.5.3.15 Ca2+ increases activity of hPADI2. 50% of maximal activity at 0.62 mM Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
3.5.3.15 Homo sapiens
-
 rheumatoid arthritis patients
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.3.15 synovium hPADI2 Homo sapiens
-
3.5.3.15 synovium hPADI4 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.3.15 benzoyl-Arg-ethyl ester + H2O hPADI2 Homo sapiens benzoyl-citrulline-ethyl ester + NH3
-
 ?
3.5.3.15 benzoyl-Arg-ethyl ester + H2O hPADI4 Homo sapiens benzoyl-citrulline-ethyl ester + NH3
-
 ?
3.5.3.15 benzoyl-Arg-methyl ester + H2O hPADI2 Homo sapiens benzoyl-citrulline-methyl ester + NH3
-
 ?
3.5.3.15 benzoyl-Arg-methyl ester + H2O hPADI4 Homo sapiens benzoyl-citrulline-methyl ester + NH3
-
 ?
3.5.3.15 benzoyl-Arg-NH2 + H2O hPADI2 Homo sapiens benzoyl-citrulline-NH2 + NH3
-
 ?
3.5.3.15 benzoyl-Arg-NH2 + H2O hPADI4 Homo sapiens benzoyl-citrulline-NH2 + NH3
-
 ?
3.5.3.15 Fibrinogen + H2O human fibrinogen, hPADI2 Homo sapiens ?
-
 ?
3.5.3.15 Fibrinogen + H2O human fibrinogen, hPADI4 Homo sapiens ?
-
 ?
3.5.3.15 filaggrin + H2O human filaggrin, hPADI2 Homo sapiens ?
-
 ?
3.5.3.15 filaggrin + H2O human filaggrin, hPADI4 Homo sapiens ?
-
 ?
3.5.3.15 tosyl-Arg-ethyl ester + H2O hPADI2 Homo sapiens tosyl-citrulline-ethyl ester + NH3
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.3.15 hPADI2
-
 Homo sapiens
3.5.3.15 hPADI4
-
 Homo sapiens
3.5.3.15 peptidylarginine deiminase
-
 Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.3.15 7
-
 hPADI2 Homo sapiens
3.5.3.15 7.5
-
 hPADI4 Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.3.15 6 10 pH 6.0: about 75% of maximal activity, pH 10.0: about 50% of maximal activity, hPADI2 Homo sapiens
3.5.3.15 6.5 9 pH 6.5: about 70% of maximal activity, pH 9.0: about 50% of maximal activity, hPADI4 Homo sapiens