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Literature summary extracted from

  • Arima, J.; Iwabuchi, M.; Hatanaka, T.
    Gene cloning and overproduction of an aminopeptidase from Streptomyces septatus TH-2, and comparison with a calcium-activated enzyme from Streptomyces griseus (2004), Biochem. Biophys. Res. Commun., 317, 531-538.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.24 gene Sgap, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli Streptomyces griseus
3.4.11.24 gene Ssap, genomic library screening, DNA and amino acid sequence determination and analysis, overexpression as secreted enzyme in Escherichia coli strain BL21(DE3) Streptomyces septatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.24 amastatin
-
 Streptomyces griseus
3.4.11.24 amastatin
-
 Streptomyces septatus
3.4.11.24 bestatin
-
 Streptomyces griseus
3.4.11.24 bestatin
-
 Streptomyces septatus
3.4.11.24 Ca2+
-
 Streptomyces griseus
3.4.11.24 Ca2+ slight inhibition at 1 mM Streptomyces septatus
3.4.11.24 Cd2+
-
 Streptomyces griseus
3.4.11.24 Cd2+
-
 Streptomyces septatus
3.4.11.24 Cu2+
-
 Streptomyces griseus
3.4.11.24 Cu2+
-
 Streptomyces septatus
3.4.11.24 Fe2+
-
 Streptomyces griseus
3.4.11.24 Fe2+
-
 Streptomyces septatus
3.4.11.24 L-leucine very low product inhibition Streptomyces griseus
3.4.11.24 L-leucine
-
 Streptomyces septatus
3.4.11.24 Mg2+
-
 Streptomyces griseus
3.4.11.24 Mg2+ slight inhibition at 0.1-1 mM Streptomyces septatus
3.4.11.24 Mn2+
-
 Streptomyces griseus
3.4.11.24 Mn2+
-
 Streptomyces septatus
3.4.11.24 additional information very low product inhibition by L-leucine Streptomyces septatus
3.4.11.24 Ni2+
-
 Streptomyces griseus
3.4.11.24 Ni2+
-
 Streptomyces septatus
3.4.11.24 Zn2+
-
 Streptomyces griseus
3.4.11.24 Zn2+
-
 Streptomyces septatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.24 0.33
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces septatus
3.4.11.24 0.34
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces septatus
3.4.11.24 0.65
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces griseus
3.4.11.24 2.3
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.24 Ca2+ activates the enzyme with some substrates, about 3fold with L-Leu-4-nitroanilide, and affects substrate specificity, e.g. decreases the activity with Lys-4-nitroanilide Streptomyces griseus
3.4.11.24 Co2+ slightly activating at 0.1-1 mM Streptomyces griseus
3.4.11.24 additional information Ca2+ and Co2+ do not affect the enzyme's substrate specificity at 0.1-1 mM Streptomyces septatus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces griseus
-
-
-
3.4.11.24 Streptomyces septatus Q75V72
-
-
3.4.11.24 Streptomyces septatus TH-2 Q75V72
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24 native enzyme by ion exchange chromatography, ammonium sulfate fractionation, and gel filtration, recombinant enzyme from Escherichia coli strain BL21(DE3) culture supernatant by ion exchange chromatography Streptomyces septatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 L-Ala-4-nitroanilide + H2O very low activity Streptomyces septatus L-Ala + 4-nitroaniline
-
 ?
3.4.11.24 L-Ala-4-nitroanilide + H2O very low activity Streptomyces septatus TH-2 L-Ala + 4-nitroaniline
-
 ?
3.4.11.24 L-Arg-4-nitroanilide + H2O low activity Streptomyces septatus L-Arg + 4-nitroaniline
-
 ?
3.4.11.24 L-Arg-4-nitroanilide + H2O low activity Streptomyces septatus TH-2 L-Arg + 4-nitroaniline
-
 ?
3.4.11.24 L-Leu-4-nitroanilide + H2O best substrate Streptomyces griseus L-Leu + 4-nitroaniline
-
 ?
3.4.11.24 L-Leu-4-nitroanilide + H2O best substrate Streptomyces septatus L-Leu + 4-nitroaniline
-
 ?
3.4.11.24 L-Leu-4-nitroanilide + H2O best substrate Streptomyces septatus TH-2 L-Leu + 4-nitroaniline
-
 ?
3.4.11.24 L-Lys-4-nitroanilide + H2O low activity Streptomyces griseus L-Lys + 4-nitroaniline
-
 ?
3.4.11.24 L-Lys-4-nitroanilide + H2O low activity Streptomyces septatus L-Lys + 4-nitroaniline
-
 ?
3.4.11.24 L-Lys-4-nitroanilide + H2O low activity Streptomyces septatus TH-2 L-Lys + 4-nitroaniline
-
 ?
3.4.11.24 L-Met-4-nitroanilide + H2O low activity Streptomyces griseus L-Met + 4-nitroaniline
-
 ?
3.4.11.24 L-Met-4-nitroanilide + H2O low activity Streptomyces septatus L-Met + 4-nitroaniline
-
 ?
3.4.11.24 L-Phe-4-nitroanilide + H2O low activity Streptomyces griseus L-Phe + 4-nitroaniline
-
 ?
3.4.11.24 L-Phe-4-nitroanilide + H2O low activity Streptomyces septatus L-Phe + 4-nitroaniline
-
 ?
3.4.11.24 L-Pro-4-nitroanilide + H2O very low activity Streptomyces septatus L-Pro + 4-nitroaniline
-
 ?
3.4.11.24 additional information no activity with L-Glu-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide Streptomyces septatus ?
-
 ?
3.4.11.24 additional information no activity with L-Glu-4-nitroanilide, L-Arg-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide, L-Ala-4-nitroanilide and L-Pro-4-nitroanilide are poor substrates Streptomyces griseus ?
-
 ?
3.4.11.24 additional information no activity with L-Glu-4-nitroanilide, D-Phe-4-nitroanilide, and D-Leu-4-nitroanilide Streptomyces septatus TH-2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 SGAP
-
 Streptomyces griseus
3.4.11.24 SSAP
-
 Streptomyces septatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.24 65
-
 in absence of Ca2+ Streptomyces griseus
3.4.11.24 75
-
-
 Streptomyces septatus
3.4.11.24 75
-
 in presence of Ca2+ Streptomyces griseus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.4.11.24 30 75 in absence of Ca2+ Streptomyces griseus
3.4.11.24 30 90
-
 Streptomyces septatus
3.4.11.24 30 90 in presence of Ca2+ Streptomyces griseus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.24 additional information
-
-
 Streptomyces griseus
3.4.11.24 additional information
-
-
 Streptomyces septatus
3.4.11.24 40 60 30 min, recombinant enzyme, stable Streptomyces septatus
3.4.11.24 70
-
 30 min, 50% loss of activity, in absence of Ca2+ Streptomyces griseus
3.4.11.24 78
-
 30 min, recombinant enzyme, 50% loss of activity Streptomyces septatus
3.4.11.24 80
-
 30 min, 50% loss of activity, in presence of Ca2+ Streptomyces griseus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.24 41.5
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces septatus
3.4.11.24 42.1
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces septatus
3.4.11.24 75.3
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in absence of Ca2+ Streptomyces griseus
3.4.11.24 223
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant enzyme, in presence of Ca2+ Streptomyces griseus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.24 8.5
-
-
 Streptomyces griseus
3.4.11.24 8.5
-
-
 Streptomyces septatus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.4.11.24 additional information
-
 pH profile Streptomyces griseus
3.4.11.24 additional information
-
 pH profile Streptomyces septatus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.24 0.000011
-
 amastatin pH 8.0, 37°C, recombinant enzyme Streptomyces griseus
3.4.11.24 0.00079
-
 amastatin pH 8.0, 37°C, recombinant enzyme Streptomyces septatus
3.4.11.24 0.0039
-
 bestatin pH 8.0, 37°C, recombinant enzyme Streptomyces griseus
3.4.11.24 0.065
-
 bestatin pH 8.0, 37°C, recombinant enzyme Streptomyces septatus
3.4.11.24 29
-
 L-leucine above, pH 8.0, 37°C, recombinant enzyme Streptomyces griseus
3.4.11.24 100
-
 L-leucine above, pH 8.0, 37°C, recombinant enzyme Streptomyces septatus