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Literature summary extracted from

  • Cheong, K.; Tang, S.; Cheong, T.; Cha, H.; Kim, J.; Park, K.
    Thermostable and alkalophilic maltogenic amylase of Bacillus thermoalkalophilus ET2 in monomer-dimer equilibrium (2005), Biocatal. Biotransform., 23, 79-87.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.133
-
 Bacillus thermoalkalophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.133 0.128
-
 beta-cyclodextrin
-
 Bacillus thermoalkalophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.133 Bacillus thermoalkalophilus
-
-
-
3.2.1.133 Bacillus thermoalkalophilus ET2
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.133 acarbose + H2O
-
 Bacillus thermoalkalophilus D-glucose + acarviosine-glucose
-
 ?
3.2.1.133 acarbose + H2O
-
 Bacillus thermoalkalophilus ET2 D-glucose + acarviosine-glucose
-
 ?
3.2.1.133 beta-cyclodextrin + H2O
-
 Bacillus thermoalkalophilus maltose + ? mainly hydrolyzed to maltose ?
3.2.1.133 beta-cyclodextrin + H2O
-
 Bacillus thermoalkalophilus ET2 maltose + ? mainly hydrolyzed to maltose ?
3.2.1.133 pullulan + H2O
-
 Bacillus thermoalkalophilus panose + ? mainly hydrolyzed to panose ?
3.2.1.133 pullulan + H2O
-
 Bacillus thermoalkalophilus ET2 panose + ? mainly hydrolyzed to panose ?
3.2.1.133 starch + H2O
-
 Bacillus thermoalkalophilus maltose + ? mainly hydrolyzed to maltose ?
3.2.1.133 starch + H2O
-
 Bacillus thermoalkalophilus ET2 maltose + ? mainly hydrolyzed to maltose ?

Subunits

EC Number Subunits Comment Organism
3.2.1.133 dimer monomer:dimer equilibrium with a molar ratio of 54:46 in 50 mM glycine-NaOH buffer pH 8.0 Bacillus thermoalkalophilus
3.2.1.133 monomer monomer:dimer equilibrium with a molar ratio of 54:46 in 50 mM glycine-NaOH buffer pH 8.0 Bacillus thermoalkalophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.133 BTMA
-
 Bacillus thermoalkalophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.133 70
-
-
 Bacillus thermoalkalophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.133 77
-
 Tm-value at pH 8 is 76.7°C Bacillus thermoalkalophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.133 8
-
-
 Bacillus thermoalkalophilus