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Literature summary extracted from

  • Harada, K.M.; Tanaka, K.; Fukuda, Y.; Hashimoto, W.; Murata, K.
    Degradation of rice bran hemicellulose by Paenibacillus sp. strain HC1: gene cloning, characterization and function of beta-D-glucosidase as an enzyme involved in degradation (2005), Arch. Microbiol., 184, 215-224.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 additional information the enzyme is induced by growth on rice bran hemicellulose, and slightly by pectin, xylan, and glucose, no effect by EDTA, L-fucose, G-glucuronic acid, D-mannose, L-rhamnose, and D-xylose Paenibacillus sp.

Application

EC Number Application Comment Organism
3.2.1.21 nutrition the enzyme is useful in rice polishing by degradation and assimilation of rice bran hemicellulose of the outer grain skin Paenibacillus sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 gene bglA, DNA and amino acid sequence determination and analysis, cDNA library construction, expression in Escherichi coli Paenibacillus sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 Ca2+ 12% inhibition at 1 mM Paenibacillus sp.
3.2.1.21 Cu2+ 79% inhibition at 1 mM Paenibacillus sp.
3.2.1.21 D-galactose 46% inhibition at 1.5 mM Paenibacillus sp.
3.2.1.21 D-glucose 76% inhibition at 1.5 mM Paenibacillus sp.
3.2.1.21 D-glucuronic acid 12% inhibition at 1.5 mM Paenibacillus sp.
3.2.1.21 D-mannose 10% inhibition at 1.5 mM Paenibacillus sp.
3.2.1.21 Fe2+ 63% inhibition at 1 mM Paenibacillus sp.
3.2.1.21 Hg2+ 99% inhibition at 1 mM Paenibacillus sp.
3.2.1.21 Mn2+ 40% inhibition at 1 mM Paenibacillus sp.
3.2.1.21 additional information no effect by EDTA, DTT, 2-mercaptoethanol, iodoacetc acid, glutathione, L-fucose, L-rhamnose, and D-xylose Paenibacillus sp.
3.2.1.21 NEM 26% inhibition at 1 mM Paenibacillus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 9.1
-
 4-nitrophenyl beta-D-glucopyranoside pH 7.0, 30°C, recombinant enzyme Paenibacillus sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.21 intracellular
-
 Paenibacillus sp. 5622
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.21 Co2+ 14% activation at 1 mM Paenibacillus sp.
3.2.1.21 additional information the enzyme is not affected by Mg2+ Paenibacillus sp.
3.2.1.21 Zn2+ 11% activation at 1 mM Paenibacillus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 50000
-
 recombinant enzyme, gel filtration Paenibacillus sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.21 rice bran hemicellulose + H2O Paenibacillus sp. the enzyme is involved in degradation ?
-
 ?
3.2.1.21 rice bran hemicellulose + H2O Paenibacillus sp. HC1 the enzyme is involved in degradation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Paenibacillus sp. Q2WGB4 isolated from soil, gene bglA
-
3.2.1.21 Paenibacillus sp. HC1 Q2WGB4 isolated from soil, gene bglA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.21 recombinant BglA 52.9fold from Escherichia coli by ammonium sulfate fractionation, ion exchange and adsorption chromatography, and gel filtration Paenibacillus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 39.4
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, enzyme expression induced by growth on rice bran hemicellulose Paenibacillus sp.
3.2.1.21 74.6
-
 purified enzyme Paenibacillus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-fucopyranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-fucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Paenibacillus sp. HC1 4-nitrophenol + beta-D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Paenibacillus sp. HC1 4-nitrophenol + beta-D-glucose
-
 ?
3.2.1.21 additional information substrate specificity, overview Paenibacillus sp. ?
-
 ?
3.2.1.21 additional information substrate specificity, overview Paenibacillus sp. HC1 ?
-
 ?
3.2.1.21 rice bran hemicellulose + H2O degradation Paenibacillus sp. ?
-
 ?
3.2.1.21 rice bran hemicellulose + H2O the enzyme is involved in degradation Paenibacillus sp. ?
-
 ?
3.2.1.21 rice bran hemicellulose + H2O degradation Paenibacillus sp. HC1 ?
-
 ?
3.2.1.21 rice bran hemicellulose + H2O the enzyme is involved in degradation Paenibacillus sp. HC1 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 50000, recombinant enzyme, SDS-PAGE Paenibacillus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 BglA
-
 Paenibacillus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 37
-
-
 Paenibacillus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 40
-
 10 min, loss of 50% activity Paenibacillus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 6 7
-
 Paenibacillus sp.