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Literature summary extracted from

  • Takatsume, Y.; Izawa, S.; Inoue, Y.
    Identification of thermostable glyoxalase I in the fission yeast Schizosaccharomyces pombe (2004), Arch. Microbiol., 181, 371-377.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.4.1.5 expression in Saccharomyces cerevisiae Schizosaccharomyces pombe

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.4.1.5 0.24
-
 Phenylglyoxal pH 7.0, 25°C Schizosaccharomyces pombe
4.4.1.5 0.8
-
 glutathione pH 7.0, 25°C Schizosaccharomyces pombe
4.4.1.5 1.6
-
 methylglyoxal pH 7.0, 25°C Schizosaccharomyces pombe

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.4.1.5 Ca2+ 20mM, 32.8% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe
4.4.1.5 Co2+ 1mM, 78.6% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe
4.4.1.5 Fe2+ 1mM, 8.4% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe
4.4.1.5 Mg2+ 20mM, 13.7% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe
4.4.1.5 Mn2+ 1mM, 186% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe
4.4.1.5 Ni2+ 1mM, 67.9% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe
4.4.1.5 Zn2+ 0.5mM, 84.7% reactivation of EDTA-treated enzyme Schizosaccharomyces pombe

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.4.1.5 34000
-
 gel filtration Schizosaccharomyces pombe

Organism

EC Number Organism UniProt Comment Textmining
4.4.1.5 Schizosaccharomyces pombe
-
 PR 109
-
4.4.1.5 Schizosaccharomyces pombe PR 109
-
 PR 109
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.4.1.5
-
 Schizosaccharomyces pombe

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.4.1.5 additional information
-
 Vmax methylglyoxal purified enzyme 2 mmol/min/mg protein, Vmax phenylglyoxal 0.197 mmol/min/mg protein, pH 7.0, 25°C Schizosaccharomyces pombe

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.4.1.5 glutathione + methylglyoxal
-
 Schizosaccharomyces pombe (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + methylglyoxal
-
 Schizosaccharomyces pombe PR 109 (R)-S-lactoylglutathione
-
 ?
4.4.1.5 glutathione + phenylglyoxal
-
 Schizosaccharomyces pombe S-mandeloylglutathione
-
 ?
4.4.1.5 glutathione + phenylglyoxal
-
 Schizosaccharomyces pombe PR 109 S-mandeloylglutathione
-
 ?

Subunits

EC Number Subunits Comment Organism
4.4.1.5 monomer 1 * 34000, SDS-PAGE Schizosaccharomyces pombe

Synonyms

EC Number Synonyms Comment Organism
4.4.1.5 Glo1
-
 Schizosaccharomyces pombe

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.4.1.5 60
-
 100% activity after 4 h incubation at 60°C, 50% inactivation after 150 min incubation at 64°C or after 60 min incubation at 65°C Schizosaccharomyces pombe

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.4.1.5 5.5 10 stable at 4°C for at least 24h Schizosaccharomyces pombe