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Literature summary extracted from

  • Picaud, S.; Olofsson, L.; Brodelius, M.; Brodelius, P.E.
    Expression, purification, and characterization of recombinant amorpha-4,11-diene synthase from Artemisia annua L (2005), Arch. Biochem. Biophys., 436, 215-226.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.24
-
 Artemisia annua
4.2.3.24 expression in Escherichia coli with His-tag Artemisia annua

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.3.24 Co2+ inhibitory above 0.5 mM Artemisia annua
4.2.3.24 Cu2+ complete inhibition Artemisia annua
4.2.3.24 Mn2+ inhibitory above 0.5 mM Artemisia annua
4.2.3.24 Ni2+
-
 Artemisia annua
4.2.3.24 Zn2+ complete inhibition Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.24 0.0006
-
 farnesyl diphosphate presence of Co2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.0007
-
 farnesyl diphosphate presence of Co2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0016
-
 farnesyl diphosphate presence of Mg2+, pH 9.5, 30°C Artemisia annua
4.2.3.24 0.002
-
 farnesyl diphosphate presence of Mg2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.0033
-
 farnesyl diphosphate presence of Mg2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0042
-
 farnesyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.008
-
 farnesyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0169
-
 geranyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0282
-
 geranyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.24 Co2+ increase in product selectivity at pH 6.5, inhibitory above 0.5 mM Artemisia annua
4.2.3.24 Mg2+
-
 Artemisia annua
4.2.3.24 Mn2+ increase in product selectivity at pH 6.5, inhibitory above 0.5 mM Artemisia annua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.24 65000
-
 x * 65000, SDS-PAGE, recombinant enzyme with His-tag Artemisia annua

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.24 Artemisia annua
-
-
-
4.2.3.24 Artemisia annua
-
 expression in Escherichia coli with His-tag
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.3.24 recombinant enzyme with his-tag Artemisia annua

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.24 (2E,6E)-farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate dditionally production of 15 different sesquiterpenoids, detailed reaction scheme ?
4.2.3.24 farnesyl diphosphate
-
 Artemisia annua amorpha-4,11-diene + diphosphate additionally production of 15 different sesquiterpenoids, detailed reaction scheme ?
4.2.3.24 Geranyl diphosphate
-
 Artemisia annua ?
-
 ?
4.2.3.24 geranyl diphosphate alpha-terpineol (39%) + ocimene (24%) + myrcene (19%) + linalool (11%) Artemisia annua alpha-terpineol + ocimene + myrcene + linalool
-
 ?
4.2.3.24 geranyl diphosphate + H2O substrate in the presence of Mn2+ but not with Mg2+ or Co2+ Artemisia annua amorpha-4,11-diene + diphosphate
-
 ?
4.2.3.24 additional information significant increased product selectivity in the presence of Mn2+ or Co2+ Artemisia annua ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.24 ? x * 65000, SDS-PAGE, recombinant enzyme with His-tag Artemisia annua

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.2.3.24 0.00034
-
 geranyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.0007
-
 geranyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.00078
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.0013
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0043
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.0068
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0108
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.015
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 0.0154
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 9.5, 30°C Artemisia annua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.24 6.5
-
-
 Artemisia annua

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.2.3.24 7.5
-
 pH-minimum, increasing enzyme activity with increasing pH above 7.5 Artemisia annua

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
4.2.3.24 0.012
-
 geranyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 0.042
-
 geranyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 1.3
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 1.9
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 2.1
-
 (2E,6E)-farnesyl diphosphate presence of Co2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 2.1
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 6.5, 30°C Artemisia annua
4.2.3.24 2.2
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 2.6
-
 (2E,6E)-farnesyl diphosphate presence of Mn2+, pH 7.5, 30°C Artemisia annua
4.2.3.24 9.7
-
 (2E,6E)-farnesyl diphosphate presence of Mg2+, pH 9.5, 30°C Artemisia annua