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Literature summary extracted from
- Differential hydrolysis of erythrocyte and mitochondrial membrane phospholipids by two phospholipase A2 isoenzymes (NK-PLA2-I and NK-PLA2-II) from the venom of the Indian monocled cobra Naja kaouthia (2004), Arch. Biochem. Biophys., 425, 1-13.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.1.4 | additional information | not inhibitory to both isoenzymes: p-methylsulfonylfluoride, tosyl-L-phenylalaninchlormethylketon, N-bromosuccinamide, tosyl-L-lysinechlormethylketon | Naja kaouthia | |
| 3.1.1.4 | p-bromophenacylbromide | i.e. pBPB, 3.3 mM, 95% inhibition of isoenzyme NK-PLA2-I, 89% inhibition of isoenzyme NK-PLA2-II | Naja kaouthia |  |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 13100 | - |
PAGE | Naja kaouthia |
| 3.1.1.4 | 13346 | - |
1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS | Naja kaouthia |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | additional information | Naja kaouthia | both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | Naja kaouthia | - |
isoenzymes NK-PLA2-I and NK-PLA2-II | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.1.4 | isoenzyme NK-PLA2-II | Naja kaouthia |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.1.1.4 | venom | - |
Naja kaouthia | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 61 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
| 3.1.1.4 | 61 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C | Naja kaouthia |
| 3.1.1.4 | 69.2 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
| 3.1.1.4 | 76.3 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
| 3.1.1.4 | 79 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylethanolamine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
| 3.1.1.4 | 89.3 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C20:4, 25°C | Naja kaouthia |
| 3.1.1.4 | 96.3 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
| 3.1.1.4 | 101.2 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylserine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
| 3.1.1.4 | 133 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C | Naja kaouthia |
| 3.1.1.4 | 153.9 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C | Naja kaouthia |
| 3.1.1.4 | 178.4 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
| 3.1.1.4 | 202.3 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
| 3.1.1.4 | 218.4 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:2, 25°C | Naja kaouthia |
| 3.1.1.4 | 220.5 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C16:0, 25°C | Naja kaouthia |
| 3.1.1.4 | 229.9 | - |
isoenzyme NK-PLA2-II, substrate phosphatidylcholine, sn-2 fatty acid C18:1, 25°C | Naja kaouthia |
| 3.1.1.4 | 243.1 | - |
isoenzyme NK-PLA2-I, substrate phosphatidylcholine, sn-2 fatty acid C18:0, 25°C | Naja kaouthia |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.1.1.4 | additional information | both isoenzymes NK-PLA2-I and NK-PLA2-II cause significantly more damage to mitochondrial membranes as compared to erythrocyte membranes | Naja kaouthia | ? | - |
? | |
| 3.1.1.4 | additional information | isoenzyme NK-PLA2-I does not discriminate between saturated and unsaturated fatty acids whereas isoenzyme NK-PLA2-II shows a preference for unsaturated fatty acids | Naja kaouthia | ? | - |
? | |
| 3.1.1.4 | phosphatidylcholine + H2O | preferred substrate for both isoenzymes, NK-PLA2-I and NK-PLA2-II | Naja kaouthia | 1-acylglycerophosphocholine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylethanolamine + H2O | - |
Naja kaouthia | 1-acylglycerophosphorylethanolamine + fatty acid | - |
? | |
| 3.1.1.4 | phosphatidylserine + H2O | - |
Naja kaouthia | 1-acylglycerophosphoserine + fatty acid | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | monomer | 1 * 13100, SDS-PAGE, 1 * 13346, MALDI-MS | Naja kaouthia |
| 3.1.1.4 | More | N-terminal amino acid sequence is identical for both isoenzymes NK-PLA2-I and NK-PLA2-II | Naja kaouthia |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.1.1.4 | NK-PLA2-I | - |
Naja kaouthia |
| 3.1.1.4 | NK-PLA2-II | - |
Naja kaouthia |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.1.4 | 100 | - |
45 min, isoenzyme NK-PLA2-I, 50% residual activity, isoenzyme NK-PLA2-II, 43% residual activity | Naja kaouthia |
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