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Literature summary extracted from

  • Mercke, P.; Bengtsson, M.; Bouwmeester, H.J.; Posthumus, M.A.; Brodelius, P.E.
    Molecular cloning, expression, and characterization of amorpha-4,11-diene synthase, a key enzyme of artemisinin biosynthesis in Artemisia annua L. (2000), Arch. Biochem. Biophys., 381, 173-180.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.24
-
 Artemisia annua

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.2.3.24 0.0009
-
 (25R)-3beta-hydroxycholest-5-en-27-oate pH 7.5 Artemisia annua

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.24 Mg2+ Km-value 0.07 mM Artemisia annua
4.2.3.24 Mn2+ Km-value 0.013 mM Artemisia annua

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.3.24 63900
-
 x * 63900, calculated Artemisia annua

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.24 Artemisia annua
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
4.2.3.24 (2E,6E)-farnesyl diphosphate = amorpha-4,11-diene + diphosphate enzyme first catalyzes a 1,6 ring closure with a subsequent 1,10 closure Artemisia annua
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.24 (25R)-3beta-hydroxycholest-5-en-27-oate
-
 Artemisia annua ?
-
 ?
4.2.3.24 farnesyl diphosphate
-
 Artemisia annua amorpha-4,7-diene + diphosphate more than 90% of product, minor products are amorpha-4,7(11)-diene, gamma-humulene, amorpha-4-en-7-ol and others ?
4.2.3.24 additional information no substrate: geranyl diphosphate Artemisia annua ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.2.3.24 ? x * 63900, calculated Artemisia annua

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.3.24 7.5 9 broad Artemisia annua