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Literature summary extracted from

  • Lee, Y.S.; Yoo, J.S.; Chung, S.Y.; Lee, Y.C.; Cho, Y.S.; Choi, Y.L.
    Cloning, purification, and characterization of chitosanase from Bacillus sp. DAU101 (2006), Appl. Microbiol. Biotechnol., 73, 113-121.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132
-
 Bacillus sp. DAU101

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Hg2+
-
 Bacillus sp. DAU101
3.2.1.132 Ni(2+)
-
 Bacillus sp. DAU101

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.132 Ca2+ 5-10 mM, activity is increased by about 1.6fold Bacillus sp. DAU101

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 27000
-
 x * 27000, SDS-PAGE Bacillus sp. DAU101
3.2.1.132 31420
-
 x * 31420, calculated from sequence Bacillus sp. DAU101

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. DAU101
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.132 proteolytic modification deletion of a signal peptide with SDS-PAGE Bacillus sp. DAU101

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Bacillus sp. DAU101

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitosan + H2O
-
 Bacillus sp. DAU101 chitobiose + chitotriose + chitotetraose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 27000, SDS-PAGE Bacillus sp. DAU101
3.2.1.132 ? x * 31420, calculated from sequence Bacillus sp. DAU101

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 50
-
-
 Bacillus sp. DAU101

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.132 7.5
-
-
 Bacillus sp. DAU101