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Literature summary extracted from

  • Ike, M.; Nagamatsu, K.; Shioya, A.; Nogawa, M.; Ogasawara, W.; Okada, H.; Morikawa, Y.
    Purification, characterization, and gene cloning of 46 kDa chitinase (Chi46) from Trichoderma reesei PC-3-7 and its expression in Escherichia coli (2006), Appl. Microbiol. Biotechnol., 71, 294-303.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 gene chi46, DNA and amino acid sequence determination and analysis, multiple regulatory proteins are involved in wild-type Chi46 expression, expression in Escherichia coli Trichoderma reesei

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.14 extracellular the enzyme is secreted to the culture medium, the enzyme sequence contains a pre-pro type signal peptide Trichoderma reesei
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 42265
-
 x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation Trichoderma reesei
3.2.1.14 46000
-
 x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Trichoderma reesei
-
 gene chi46
-
3.2.1.14 Trichoderma reesei PC-3-7
-
 gene chi46
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14 native enzyme from the culture medium Trichoderma reesei

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.14 Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins exo-type substrate cleavage mechanism Trichoderma reesei
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.14 mycelium
-
 Trichoderma reesei
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 chitin + H2O colloidal and ethylene glycol chitin, exo-type cleavage mode Trichoderma reesei GlcNAc2 + ? main product from insoluble colloidal chitin ?
3.2.1.14 chitin + H2O colloidal and ethylene glycol chitin, exo-type cleavage mode Trichoderma reesei PC-3-7 GlcNAc2 + ? main product from insoluble colloidal chitin ?
3.2.1.14 chitosan 7B + H2O the activity increases with increasing acetylation of the substrate Trichoderma reesei ?
-
 ?
3.2.1.14 chitosan 7B + H2O the activity increases with increasing acetylation of the substrate Trichoderma reesei PC-3-7 ?
-
 ?
3.2.1.14 additional information multiple regulatory proteins are involved in Chi46 expression Trichoderma reesei ?
-
 ?
3.2.1.14 additional information multiple regulatory proteins are involved in Chi46 expression Trichoderma reesei PC-3-7 ?
-
 ?
3.2.1.14 N-acetylchito-oligosaccharides + H2O endo-type cleavage mode Trichoderma reesei ?
-
 ?
3.2.1.14 N-acetylchito-oligosaccharides + H2O endo-type cleavage mode Trichoderma reesei PC-3-7 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 46000, SDS-PAGE, x * 42265, Chi46, amino acid sequence calculation Trichoderma reesei
3.2.1.14 additional information the enzyme sequence contains a pre-pro type signal peptide Trichoderma reesei

Synonyms

EC Number Synonyms Comment Organism
3.2.1.14 Chi46
-
 Trichoderma reesei