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Literature summary extracted from

  • Delattre, C.; Michaud, P.; Keller, C.; Elboutachfaiti, R.; Beven, L.; Courtois, B.; Courtois, J.
    Purification and characterization of a novel glucuronan lyase from Trichoderma sp. GL2 (2006), Appl. Microbiol. Biotechnol., 70, 437-443.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.2.2.14 Cu2+ 1 mM, 81% inhibition Trichoderma sp.
4.2.2.14 Fe2+ 1 mM, 19% inhibition Trichoderma sp.
4.2.2.14 Ni2+ 1 mM, 60% inhibition Trichoderma sp.
4.2.2.14 Rb+ 1 mM, 10% inhibition Trichoderma sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
4.2.2.14 extracellular
-
 Trichoderma sp.
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.2.14 Ca2+ 1 mM, enhances activity 4.56fold Trichoderma sp.
4.2.2.14 Co2+ 1 mM, enhances activity 1.5fold Trichoderma sp.
4.2.2.14 Li+ 1 mM, enhances activity 4.4fold Trichoderma sp.
4.2.2.14 Mg2+ 1 mM, enhances activity 2.5fold Trichoderma sp.
4.2.2.14 Mn2+ 1 mM, enhances activity 2.2fold Trichoderma sp.
4.2.2.14 additional information enzyme appears not to be really affected by ionic strength variations. The optimum is 300 mM concentration of potassium acetate buffer Trichoderma sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.2.2.14 27000
-
 x * 27000, SDS-PAGE Trichoderma sp.

Organism

EC Number Organism UniProt Comment Textmining
4.2.2.14 Trichoderma sp.
-
 GL2
-
4.2.2.14 Trichoderma sp. GL2
-
 GL2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.2.2.14
-
 Trichoderma sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.2.2.14 162
-
-
 Trichoderma sp.

Subunits

EC Number Subunits Comment Organism
4.2.2.14 ? x * 27000, SDS-PAGE Trichoderma sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
4.2.2.14 55
-
-
 Trichoderma sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4.2.2.14 35
-
 1 h, stable below Trichoderma sp.
4.2.2.14 40
-
 1 h, 90% loss of activity Trichoderma sp.
4.2.2.14 60
-
 1 h, complete inactivation Trichoderma sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.2.2.14 5.5
-
-
 Trichoderma sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.2.2.14 4 8 30 min, stable Trichoderma sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.2.2.14 Trichoderma sp. isoelectric focusing
-
 6.95