EC Number | Application | Comment | Organism |
---|---|---|---|
3.2.1.21 | biotechnology | the enzyme might be useful as biocatalyst in the synthesis of glyco-conjugates, overview | Schwanniomyces etchellsii |
EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.21 | expression in Escherichia coli strain JM109 | Schwanniomyces etchellsii |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.2.1.21 | diethyldicarbonate | binds to the active site, 4-nitrophenyl beta-D-glucoside partially protects against inactivation | Schwanniomyces etchellsii |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 83000 | - |
2 * 83000, recombinant enzyme | Schwanniomyces etchellsii |
3.2.1.21 | 176000 | - |
recombinant enzyme | Schwanniomyces etchellsii |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.21 | Schwanniomyces etchellsii | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.21 | recombinant enzyme from Escherichia coli strain JM109 | Schwanniomyces etchellsii |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.21 | celloheptaose + 6 H2O = 7 beta-D-glucose | the enzyme contains a His residue which is important for catalytic activity | Schwanniomyces etchellsii |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | 0.77 | - |
purified recombinant enzyme, substrate sophorose | Schwanniomyces etchellsii |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.21 | 4-nitrophenyl beta-D-glucoside + H2O | - |
Schwanniomyces etchellsii | 4-nitrophenol + beta-D-glucose | - |
? | |
3.2.1.21 | amygdalin + H2O | - |
Schwanniomyces etchellsii | ? | - |
? | |
3.2.1.21 | additional information | the enzyme shows broad substrate specificity hydrolyzing beta-linked gluco-disaccharides and oligosaccharides, and transglycosylation activity forming beta-linked di- and oligosaccharides | Schwanniomyces etchellsii | ? | - |
? | |
3.2.1.21 | salicin + H2O | - |
Schwanniomyces etchellsii | ? | - |
? | |
3.2.1.21 | sophorose + H2O | - |
Schwanniomyces etchellsii | 2 D-glucose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.21 | dimer | 2 * 83000, recombinant enzyme | Schwanniomyces etchellsii |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.21 | beta-glucosidase II | - |
Schwanniomyces etchellsii |
3.2.1.21 | More | the enzyme belongs to the glycosyl hydrolase family 3 | Schwanniomyces etchellsii |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.21 | additional information | - |
the enzyme is thermotolerant | Schwanniomyces etchellsii |