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Literature summary extracted from

  • Arima, J.; Uesugi, Y.; Iwabuchi, M.; Hatanaka, T.
    Alteration of leucine aminopeptidase from Streptomyces septatus TH-2 to phenylalanine aminopeptidase by site-directed mutagenesis (2005), Appl. Environ. Microbiol., 71, 7229-7235.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.24 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) Streptomyces septatus

Protein Variants

EC Number Protein Variants Comment Organism
3.4.11.24 F221A site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme Streptomyces septatus
3.4.11.24 F221G site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme Streptomyces septatus
3.4.11.24 F221S site-directed mutagenesis, the mutant shows altered substrate specificity compared to the wild-type enzyme Streptomyces septatus
3.4.11.24 additional information alteration of leucine aminopeptidase to phenylalanine aminopeptidase by site-directed mutagenesis Streptomyces septatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.11.24 additional information
-
 additional information kinetics of recombinant wild-type and F221 mutant enzymes Streptomyces septatus
3.4.11.24 0.25
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 0.34
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 0.36
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 0.44
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 0.68
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 1.85
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 6.9
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 6.98
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 7.68
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 8.31
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 12.8
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 15.6
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 15.8
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 16.1
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 24.2
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 42.7
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.24 Streptomyces septatus Q75V72 gene Ssap
-
3.4.11.24 Streptomyces septatus TH-2 Q75V72 gene Ssap
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.24 recombinant wild-type and mutant enzymes from Escherichia coli strain Bl21(DE3) Streptomyces septatus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.11.24 additional information
-
-
 Streptomyces septatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.24 L-Leu-4-nitroanilide + H2O preferred substrate of the wild-type enzyme Streptomyces septatus L-Leu + 4-nitroaniline
-
 ?
3.4.11.24 L-Leu-4-nitroanilide + H2O preferred substrate of the wild-type enzyme Streptomyces septatus TH-2 L-Leu + 4-nitroaniline
-
 ?
3.4.11.24 L-Leu-NH2 + H2O
-
 Streptomyces septatus L-Leu + NH3
-
 ?
3.4.11.24 L-Leu-NH2 + H2O
-
 Streptomyces septatus TH-2 L-Leu + NH3
-
 ?
3.4.11.24 L-Leu-O-methyl ester + H2O
-
 Streptomyces septatus L-Leu + methanol
-
 ?
3.4.11.24 L-Leu-O-methyl ester + H2O
-
 Streptomyces septatus TH-2 L-Leu + methanol
-
 ?
3.4.11.24 L-Phe-4-nitroanilide + H2O
-
 Streptomyces septatus L-Phe + 4-nitroaniline
-
 ?
3.4.11.24 L-Phe-4-nitroanilide + H2O
-
 Streptomyces septatus TH-2 L-Phe + 4-nitroaniline
-
 ?
3.4.11.24 L-Phe-NH2 + H2O
-
 Streptomyces septatus L-Phe + NH3
-
 ?
3.4.11.24 L-Phe-O-methyl ester + H2O
-
 Streptomyces septatus L-Phe + methanol
-
 ?
3.4.11.24 additional information substrate specificities of recombinant wild-type and F221 mutant enzymes, overview Streptomyces septatus ?
-
 ?
3.4.11.24 additional information substrate specificities of recombinant wild-type and F221 mutant enzymes, overview Streptomyces septatus TH-2 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.11.24 leucine aminopeptidase
-
 Streptomyces septatus
3.4.11.24 SSAP
-
 Streptomyces septatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.11.24 37
-
 assay at Streptomyces septatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.11.24 0.64
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 10.7
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 17.6
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 19.3
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 31.7
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 34
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 43.8
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 54.3
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 64.2
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 65.9
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 104
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 108
-
 L-Leu-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 115
-
 L-Phe-NH2 pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 142
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant wild-type enzyme Streptomyces septatus
3.4.11.24 155
-
 L-Leu-NH2 pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 169
-
 L-Leu-O-methyl ester pH 8.0, 37°C, recombinant mutant F221I Streptomyces septatus
3.4.11.24 224
-
 L-Phe-O-methyl ester pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus
3.4.11.24 229
-
 L-Phe-4-nitroanilide pH 8.0, 37°C, recombinant mutant F221A Streptomyces septatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.24 8
-
 assay at Streptomyces septatus