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Literature summary extracted from

  • Misono, H.; Maeda, H.; Tuda, K.; Ueshima, S.; Miyazaki, N.; Nagata, S.
    Characterization of an inducible phenylserine aldolase from Pseudomonas putida 24-1 (2005), Appl. Environ. Microbiol., 71, 4602-4609.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.1.2.26 overexpression in Escherichia coli Pseudomonas putida

Protein Variants

EC Number Protein Variants Comment Organism
4.1.2.26 K213Q loss of activity, disappearance of absorption maximum at 420 nm Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.2.26 3-(ethylamino)phenol competitive against DL-threo-3-phenylserine Pseudomonas putida
4.1.2.26 D-cycloserine 1 mM, 98% inhibition Pseudomonas putida
4.1.2.26 DL-3-hydroxy-n-butyrate 20 mM, 30% inhibition Pseudomonas putida
4.1.2.26 DL-3-hydroxynorvaline 20 mM, 49% inhibition Pseudomonas putida
4.1.2.26 DL-3-hydroxyphenylethylamine 20 mM, 79% inhibition Pseudomonas putida
4.1.2.26 hydroxylamine 1 mM, 97% inhibition Pseudomonas putida
4.1.2.26 L-3-phenyllactate 10 mM, 39% inhibition Pseudomonas putida
4.1.2.26 phenylhydrazine 1 mM, 52% inhibition Pseudomonas putida
4.1.2.26 Semicarbazide 1 mM, 97% inhibition Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.2.26 1.3
-
 L-threo-3-phenylserine pH 8.5, 30°C Pseudomonas putida
4.1.2.26 4.6
-
 L-erythro-3-phenylserine pH 8.5, 30°C Pseudomonas putida
4.1.2.26 22
-
 L-allo-Thr pH 8.5, 30°C Pseudomonas putida
4.1.2.26 29
-
 L-Thr pH 8.5, 30°C Pseudomonas putida

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.2.26 37400
-
 6 * 37400, calculation from nucleotide sequence Pseudomonas putida
4.1.2.26 38000
-
 6 * 38000, SDS-PAGE Pseudomonas putida
4.1.2.26 210000
-
 gel filtration Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.2.26 additional information Pseudomonas putida inducible enzyme ?
-
 ?
4.1.2.26 additional information Pseudomonas putida 24. Jan inducible enzyme ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.2.26 Pseudomonas putida Q59IT3 strain 24-1
-
4.1.2.26 Pseudomonas putida 24. Jan Q59IT3 strain 24-1
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.2.26 recombinant Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.2.26 4.3
-
 reaction with glycine and benzaldehyde Pseudomonas putida
4.1.2.26 300
-
 cleavage of L-threo-3-phenylserine Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
4.1.2.26 -20°C, pH 7.2, 10 mM TES buffer, 0.01% 2-mercaptoethanol, 0.05 mM pyridoxal 5'-phosphate, 30% glycerol, stable for several months Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.2.26 glycine + benzaldehyde
-
 Pseudomonas putida L-threo-3-phenylserine + L-erythro-3-phenylserine
-
 r
4.1.2.26 glycine + benzaldehyde
-
 Pseudomonas putida 24. Jan L-threo-3-phenylserine + L-erythro-3-phenylserine
-
 r
4.1.2.26 L-allo-Thr
-
 Pseudomonas putida glycine + acetaldehyde
-
 ?
4.1.2.26 L-erythro-3-phenylserine no activity with D-erythro-3-phenylserine Pseudomonas putida glycine + benzaldehyde
-
 r
4.1.2.26 L-Thr
-
 Pseudomonas putida glycine + acetaldehyde
-
 ?
4.1.2.26 L-Thr
-
 Pseudomonas putida 24. Jan glycine + acetaldehyde
-
 ?
4.1.2.26 L-threo-3-phenylserine no activity with D-threo-3-phenylserine Pseudomonas putida glycine + benzaldehyde
-
 r
4.1.2.26 L-threo-3-phenylserine no activity with D-threo-3-phenylserine Pseudomonas putida 24. Jan glycine + benzaldehyde
-
 r
4.1.2.26 additional information inducible enzyme Pseudomonas putida ?
-
 ?
4.1.2.26 additional information no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine Pseudomonas putida ?
-
 ?
4.1.2.26 additional information inducible enzyme Pseudomonas putida 24. Jan ?
-
 ?
4.1.2.26 additional information no activity with D-Ser, L-Ser, D-Thr, D-allo-Thr, DL-3-hydroxyphenylethylamine, DL-2-amino-3-phenyl-n-butanoate, L-3-phenyllactate, DL-homoserine, D-glucosaminate, D-mannosamine, D-glucosamine, or D-galactosamine Pseudomonas putida 24. Jan ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.2.26 hexamer 6 * 38000, SDS-PAGE Pseudomonas putida
4.1.2.26 hexamer 6 * 37400, calculation from nucleotide sequence Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
4.1.2.26 580
-
 L-Thr pH 8.5, 30°C Pseudomonas putida
4.1.2.26 1300
-
 L-allo-Thr pH 8.5, 30°C Pseudomonas putida
4.1.2.26 2300
-
 L-threo-3-phenylserine pH 8.5, 30°C Pseudomonas putida
4.1.2.26 7900
-
 L-erythro-3-phenylserine pH 8.5, 30°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.2.26 7.5
-
 reaction with glycine and benzyldehyde Pseudomonas putida
4.1.2.26 8.5
-
 reaction with L-threo-3-phenylserine Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
4.1.2.26 6.5 9.5 30°C, 10 min, stable Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.2.26 pyridoxal 5'-phosphate 0.7 mol of pyridoxal 5'-phosphate per mol of subunit, K213 of the enzyme probably forms a Schiff base with pyridoxal 5'-phosphate Pseudomonas putida

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
4.1.2.26 4.1
-
 3-(ethylamino)phenol
-
 Pseudomonas putida