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Literature summary extracted from

  • Choi, Y.J.; Kim, E.J.; Piao, Z.; Yun, Y.C.; Shin, Y.C.
    Purification and characterization of chitosanase from Bacillus sp. strain KCTC 0377BP and its application for the production of chitosan oligosaccharides (2004), Appl. Environ. Microbiol., 70, 4522-4531.
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
3.2.1.132 synthesis valuable enzyme for the commercial production of chitosan oligosaccharides and other chitosan hydrolysates Bacillus sp. (in: Bacteria)

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.132
-
 Bacillus sp. (in: Bacteria)

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.132 Hg2+ 1 mM, 68% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 KCl 0.4 M, 50% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 Mn2+ 1 mM, 69% inhibition Bacillus sp. (in: Bacteria)
3.2.1.132 NaCl 0.4 M, 50% inhibition Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.132 45000
-
 x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.132 Bacillus sp. (in: Bacteria) Q9ALZ1
-
-
3.2.1.132 Bacillus sp. (in: Bacteria) KCTC 0377BP Q9ALZ1
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.132
-
 Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.132 1700
-
-
 Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.132 chitoheptaose + H2O 23.8% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitoheptaose + H2O 23.8% of the activity with chitosan Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?
3.2.1.132 chitohexaose + H2O 20.0% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitopentaose + H2O 16.8% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitopentaose + H2O 16.8% of the activity with chitosan Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?
3.2.1.132 chitosan + H2O activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate Bacillus sp. (in: Bacteria) chitosan oligosaccharides
-
 ?
3.2.1.132 chitosan + H2O activity of the enzyme increases with an increase of the degrees of deacetylation of the chitosan substrate Bacillus sp. (in: Bacteria) KCTC 0377BP chitosan oligosaccharides
-
 ?
3.2.1.132 chitotetraose + H2O 15.0% of the activity with chitosan Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 chitotetraose + H2O 15.0% of the activity with chitosan Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?
3.2.1.132 additional information no hydrolysis of chitobiose and chitotriose Bacillus sp. (in: Bacteria) ?
-
 ?
3.2.1.132 additional information no hydrolysis of chitobiose and chitotriose Bacillus sp. (in: Bacteria) KCTC 0377BP ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.132 ? x * 45000, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.132 60
-
-
 Bacillus sp. (in: Bacteria)

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.132 45
-
 24 h, enzyme retains full activity Bacillus sp. (in: Bacteria)
3.2.1.132 50
-
 half-life: 5 h Bacillus sp. (in: Bacteria)
3.2.1.132 55
-
 half-life: 10 min Bacillus sp. (in: Bacteria)
3.2.1.132 60
-
 half-life: 5 min Bacillus sp. (in: Bacteria)

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.132 2
-
 1 h, complete inactivation Bacillus sp. (in: Bacteria)
3.2.1.132 4 8 1 h, stable Bacillus sp. (in: Bacteria)