EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.10 | expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain M15 | Geobacillus kaustophilus |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
3.4.11.10 | M224L | site-directed mutagenesis, the mutant enzyme shows similar oxidative stability with H2O2 as the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M282L | site-directed mutagenesis, the mutant enzyme shows over 43% reduced activity compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M285L | site-directed mutagenesis, the mutant enzyme shows over 43% reduced activity compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M289L | site-directed mutagenesis, the mutant enzyme shows over 43% reduced activity compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M299L | site-directed mutagenesis, the mutant enzyme shows increased oxidative stability with H2O2 compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M321L | site-directed mutagenesis, the mutant enzyme shows over 43% reduced activity and reduced oxidative stability with H2O2 compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M400L | site-directed mutagenesis, the mutant enzyme shows 191% increased activity and increased catalytic efficiency compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M426L | site-directed mutagenesis, the mutant enzyme shows 79% increased activity and increased catalytic efficiency compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M445L | site-directed mutagenesis, the mutant enzyme shows 313% increased activity and increased catalytic efficiency compared to the wild-type enzyme | Geobacillus kaustophilus |
3.4.11.10 | M485L | site-directed mutagenesis, the mutant enzyme shows 103% increased activity and increased catalytic efficiency compared to the wild-type enzyme | Geobacillus kaustophilus |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.10 | H2O2 | the enzyme is sensitive against oxidative damage by H2O2 | Geobacillus kaustophilus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | additional information | - |
additional information | kinetics of wild-type and methionine mutant enzymes, overview | Geobacillus kaustophilus | |
3.4.11.10 | 2.1 | - |
L-Leu-4-nitroanilide | pH 8.0, 55°C, recombinant wild-type enzyme | Geobacillus kaustophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.10 | Geobacillus kaustophilus | - |
- |
- |
EC Number | Oxidation Stability | Organism |
---|---|---|
3.4.11.10 | the enzyme is sensitive against oxidative damage by H2O2 | Geobacillus kaustophilus |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.10 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain M15 by nickel affinity chromatography | Geobacillus kaustophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 91.6 | - |
purified recombinant wild-type enzyme | Geobacillus kaustophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.10 | L-Leu-4-nitroanilide + H2O | - |
Geobacillus kaustophilus | L-Leu + 4-nitroaniline | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.10 | LAP | - |
Geobacillus kaustophilus |
3.4.11.10 | leucine aminopeptidase | - |
Geobacillus kaustophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 55 | - |
assay at | Geobacillus kaustophilus |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.11.10 | 6.31 | - |
L-Leu-4-nitroanilide | pH 8.0, 55°C, recombinant wild-type enzyme | Geobacillus kaustophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 8 | - |
assay at | Geobacillus kaustophilus |