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Literature summary extracted from

  • Kim, H.J.; Kim, D.Y.; Nam, J.S.; Bae, K.S.; Rhee, Y.H.
    Characterization of an extracellular medium-chain-length poly(3-hydroxyalkanoate) depolymerase from Streptomyces sp. KJ-72 (2003), Antonie van Leeuwenhoek, 83, 183-189.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.1.1.76 additional information the enzyme activity is not affected by DTT Streptomyces sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.76 Acetic anhydride 45% inhibition at 5 mM Streptomyces sp.
3.1.1.76 diisopropyl fluorophosphate 83% inhibition at 5 mM Streptomyces sp.
3.1.1.76 additional information no inhibition by cyanamide, iodoacetamide, PMSF, sodium azide, EDTA, and 4-hydroxymercuribenzoate, the enzyme activity is not affected by DTT Streptomyces sp.
3.1.1.76 N-bromosuccimide 45% inhibition at 1 mM Streptomyces sp.
3.1.1.76 Triton X-100 92% inhibition at 0.5 mM Streptomyces sp.
3.1.1.76 Tween 80 58% inhibition at 0.5 mM Streptomyces sp.

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.1.76 extracellular the enzyme is secreted Streptomyces sp.
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.76 27160
-
 MALDI-TOF/mass spectrometry Streptomyces sp.
3.1.1.76 27164
-
 1 * 28000, SDS-PAGE, 1 * 27164, MALDI-TOF/mass spectrometry Streptomyces sp.
3.1.1.76 28000
-
 1 * 28000, SDS-PAGE, 1 * 27164, MALDI-TOF/mass spectrometry Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.76 Streptomyces sp.
-
-
-
3.1.1.76 Streptomyces sp. KJ-72
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.1.1.76 no glycoprotein
-
 Streptomyces sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.76 12.6fold to homogeneity by DEAE ion exchange chromatography and gel filtration Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.76 17.7
-
 purified enzyme Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.76 additional information the enzyme shows no lipase activity, the enzyme is active with several 4-nitrophenyl esters of fatty acids Streptomyces sp. ?
-
 ?
3.1.1.76 additional information the enzyme shows no lipase activity, the enzyme is active with several 4-nitrophenyl esters of fatty acids Streptomyces sp. KJ-72 ?
-
 ?
3.1.1.76 poly(3-hydroxyheptanoate) + H2O
-
 Streptomyces sp. di(3-hydroxyheptanoate) main product, small amount of monomeric product, no oligomers, identification by GC/MS ?
3.1.1.76 poly(3-hydroxyheptanoate) + H2O
-
 Streptomyces sp. KJ-72 di(3-hydroxyheptanoate) main product, small amount of monomeric product, no oligomers, identification by GC/MS ?

Subunits

EC Number Subunits Comment Organism
3.1.1.76 monomer 1 * 28000, SDS-PAGE, 1 * 27164, MALDI-TOF/mass spectrometry Streptomyces sp.

Synonyms

EC Number Synonyms Comment Organism
3.1.1.76 MCL-PHA depolymerase
-
 Streptomyces sp.
3.1.1.76 medium-chain-length poly(3-hydroxyalkanoate) depolymerase
-
 Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.76 50
-
-
 Streptomyces sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.1.76 40
-
 stable at Streptomyces sp.
3.1.1.76 60
-
 1 h, almost complete inactivation Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.76 8.7
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.76 7.5 9 70% at pH 7.5 and at pH 9.0 Streptomyces sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.1.76 6 10 fairly stable over this range Streptomyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.76 Streptomyces sp. isoelectric focusing
-
 4.7