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Literature summary extracted from

  • Voha, C.; Docquier, J.D.; Rossolini, G.M.; Fosse, T.
    Genetic and biochemical characterization of FUS-1 (OXA-85), a narrow-spectrum class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum (2006), Antimicrob. Agents Chemother., 50, 2673-2679.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.2.6 expressed in Escherichia coli BL21(DE3) cells Fusobacterium nucleatum
3.5.2.6 expression in Escherichia coli Fusobacterium nucleatum

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanate
-
Fusobacterium nucleatum
3.5.2.6 clavulanic acid poorly inactivated by clavulanate Fusobacterium nucleatum
3.5.2.6 NaCl poorly inactivated by NaCl Fusobacterium nucleatum
3.5.2.6 tazobactam
-
Fusobacterium nucleatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.01
-
benzylpenicillin at 30°C Fusobacterium nucleatum
3.5.2.6 0.023
-
CENTA at 30°C Fusobacterium nucleatum
3.5.2.6 0.036
-
Oxacillin at 30°C Fusobacterium nucleatum
3.5.2.6 0.047
-
cephalothin at 30°C Fusobacterium nucleatum
3.5.2.6 0.076
-
ampicillin at 30°C Fusobacterium nucleatum
3.5.2.6 10
-
benzylpenicillin 30°C Fusobacterium nucleatum
3.5.2.6 36
-
Oxacillin 30°C Fusobacterium nucleatum
3.5.2.6 47
-
cephalothin 30°C Fusobacterium nucleatum
3.5.2.6 76
-
ampicillin 30°C Fusobacterium nucleatum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.2.6 28910
-
electrospray mass spectrometry, corresponding to the cleavage of the N-terminal extremity after residue Phe6 Fusobacterium nucleatum
3.5.2.6 29050
-
electrospray mass spectrometry, corresponding to the cleavage of the N-terminal extremity after residue Met5 Fusobacterium nucleatum
3.5.2.6 30000
-
gel filtration Fusobacterium nucleatum

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Fusobacterium nucleatum Q6XL56 subsp. polymorphum NI61
-
3.5.2.6 Fusobacterium nucleatum Q6XL56 Fusobacterium nucleatum polymorphum strain NI61
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.2.6
-
Fusobacterium nucleatum
3.5.2.6 Q sepharose column chromatography Fusobacterium nucleatum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.2.6 4.8
-
with 1 mM ampicillin as substrate, at 30°C Fusobacterium nucleatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
Fusobacterium nucleatum (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 benzylpenicillin + H2O
-
Fusobacterium nucleatum (2R,4S)-2-[(R)-carboxy(2-phenylacetamido)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 cefuroxime + H2O
-
Fusobacterium nucleatum (2R)-5-[(carbamoyloxy)methyl]-2-[(R)-carboxy{[(2Z)-2-(furan-2-yl)-2-(methoxyimino)acetyl]amino}methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 CENTA + H2O
-
Fusobacterium nucleatum (2R)-5-{[(3-carboxy-4-nitrophenyl)sulfanyl]methyl}-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 cephalothin + H2O
-
Fusobacterium nucleatum (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
?
3.5.2.6 additional information ticarcillin and cefuroxime are weakly recognized by the enzyme, while cefotaxime, ceftazidime, and cefepime are not hydrolyzed Fusobacterium nucleatum ?
-
?
3.5.2.6 oxacillin + H2O
-
Fusobacterium nucleatum (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?
3.5.2.6 ticarcillin + H2O
-
Fusobacterium nucleatum (2R,4S)-2-[(R)-carboxy[[(2R)-2-carboxy-2-(thiophen-3-yl)acetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 class D beta-lactamase
-
Fusobacterium nucleatum
3.5.2.6 FUS-1
-
Fusobacterium nucleatum
3.5.2.6 OXA-85
-
Fusobacterium nucleatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.42
-
CENTA at 30°C Fusobacterium nucleatum
3.5.2.6 4.1
-
cephalothin 30°C Fusobacterium nucleatum
3.5.2.6 4.1
-
cephalothin at 30°C Fusobacterium nucleatum
3.5.2.6 45
-
ampicillin 30°C Fusobacterium nucleatum
3.5.2.6 45
-
ampicillin at 30°C Fusobacterium nucleatum
3.5.2.6 780
-
Oxacillin 30°C Fusobacterium nucleatum
3.5.2.6 780
-
Oxacillin at 30°C Fusobacterium nucleatum
3.5.2.6 830
-
benzylpenicillin 30°C Fusobacterium nucleatum
3.5.2.6 830
-
benzylpenicillin at 30°C Fusobacterium nucleatum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.5.2.6 Fusobacterium nucleatum isoelectric focusing
-
5.3

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.5.2.6 0.0025
-
at 30°C Fusobacterium nucleatum tazobactam
3.5.2.6 0.5
-
at 30°C Fusobacterium nucleatum clavulanic acid