Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Keith, K.E.; Oyston, P.C.; Crossett, B.; Fairweather, N.F.; Titball, R.W.; Walsh, T.R.; Brown, K.A.
    Functional characterization of OXA-57, a class D beta-lactamase from Burkholderia pseudomallei (2005), Antimicrob. Agents Chemother., 49, 1639-1641.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
3.5.2.6 D170N increase in Ki-value for clavulanic acid, little difference in the hydrolytic profile of beta-lactams compared to wild-type,no ceftazidime hydrolysis Burkholderia pseudomallei
3.5.2.6 K232N increase in Ki-value for clavulanic acid, little difference in the hydrolytic profile of beta-lactams compared to wild-type Burkholderia pseudomallei
3.5.2.6 S104P mutant enzyme shows no beta-lactam hydrolytic activity Burkholderia pseudomallei

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.2.6 clavulanic acid competitive Burkholderia pseudomallei
3.5.2.6 additional information no inhibition of wild-type enzyme and mutant enzymes D170N and K232N by NaCl up to 1 M Burkholderia pseudomallei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.2.6 0.00948
-
 piperacillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.0132
-
 nitrocefin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.0213
-
 piperacillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.0221
-
 piperacillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.0287
-
 Cloxacillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.0295
-
 penicillin G wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.0372
-
 Oxacillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.0389
-
 ampicillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.0407
-
 Oxacillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.0466
-
 penicillin G mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.0504
-
 nitrocefin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.0554
-
 cephalothin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.0563
-
 penicillin G mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.0719
-
 ampicillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.0979
-
 cephaloridine wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.1
-
 cephalothin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.157
-
 ampicillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.163
-
 cephalothin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.166
-
 Cloxacillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.179
-
 nitrocefin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.18
-
 Cloxacillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.194
-
 Oxacillin mutant enzyme K232N Burkholderia pseudomallei

Organism

EC Number Organism UniProt Comment Textmining
3.5.2.6 Burkholderia pseudomallei
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.2.6 ampicillin + H2O
-
 Burkholderia pseudomallei (2R,4S)-2-[(R)-[[(2R)-2-amino-2-phenylacetyl]amino](carboxy)methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 cephaloridine + H2O
-
 Burkholderia pseudomallei (2R)-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-5-(pyridinium-1-ylmethyl)-3,6-dihydro-2H-1,3-thiazine-4-carboxylate
-
 ?
3.5.2.6 cephalothin + H2O
-
 Burkholderia pseudomallei (2R)-5-[(acetyloxy)methyl]-2-[(R)-carboxy[(thiophen-2-ylacetyl)amino]methyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 cloxacillin + H2O
-
 Burkholderia pseudomallei (2R,4S)-2-[(R)-carboxy{[3-(2-chlorophenyl)-5-methyl-1,2-oxazole-4-carbonyl]amino}methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 nitrocefin + H2O
-
 Burkholderia pseudomallei (2R)-2-{(R)-carboxy[2-(thiophen-2-yl)acetamido]methyl}-5-[(E)-2-(2,4-dinitrophenyl)ethenyl]-3,6-dihydro-2H-1,3-thiazine-4-carboxylic acid
-
 ?
3.5.2.6 oxacillin + H2O
-
 Burkholderia pseudomallei (2R,4S)-2-{(R)-carboxy[(5-methyl-3-phenyl-1,2-oxazole-4-carbonyl)amino]methyl}-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 penicillin G + H2O
-
 Burkholderia pseudomallei (2R,4S)-2-[(R)-carboxy[(phenylacetyl)amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?
3.5.2.6 piperacillin + H2O
-
 Burkholderia pseudomallei (2R,4S)-2-[(R)-carboxy[[(2R)-2-[[(4-ethyl-2,3-dioxopiperazin-1-yl)carbonyl]amino]-2-phenylacetyl]amino]methyl]-5,5-dimethyl-1,3-thiazolidine-4-carboxylic acid
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.5.2.6 OXA-57
-
 Burkholderia pseudomallei

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.5.2.6 0.28
-
 cephalothin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.4
-
 penicillin G wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.51
-
 Cloxacillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.54
-
 ampicillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 0.61
-
 penicillin G mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 0.65
-
 cephalothin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.75
-
 penicillin G mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 0.86
-
 cephalothin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 1.04
-
 Cloxacillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 1.12
-
 Cloxacillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 1.23
-
 Oxacillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 1.42
-
 ampicillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 2.11
-
 piperacillin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 2.12
-
 ampicillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 2.37
-
 Oxacillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 3.07
-
 Oxacillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 4.31
-
 piperacillin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 5.7
-
 piperacillin mutant enzyme D170N Burkholderia pseudomallei
3.5.2.6 13
-
 cephaloridine wild-type enzyme Burkholderia pseudomallei
3.5.2.6 29.7
-
 nitrocefin mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 63
-
 nitrocefin wild-type enzyme Burkholderia pseudomallei
3.5.2.6 79.5
-
 nitrocefin mutant enzyme D170N Burkholderia pseudomallei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.5.2.6 3.4
-
 clavulanic acid wild-type enzyme Burkholderia pseudomallei
3.5.2.6 3.9
-
 clavulanic acid mutant enzyme K232N Burkholderia pseudomallei
3.5.2.6 4.6
-
 clavulanic acid mutant enzyme D170N Burkholderia pseudomallei