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Literature summary extracted from

  • Lendeckel, U.; Bukowska, A.; Laettig, J.H.; Brandt, W.
    Alanyl-aminopeptidases in human T cells. Structures and functions (2004), Aminopeptidases in Biology and Disease (Hooper, N. M. ; Lendechel, U. , eds. ), 2, 201-227.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.11.2 additional information the enzyme is induced by interleukin-4, IFNgamma, CD3, CD28, and concanavalin A Homo sapiens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.2 expression analysis Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.11.2 actinonin IC50: 0.000075 mM, kidney enzyme Homo sapiens
3.4.11.2 bestatin inhibits enzyme activity and cell growth, e.g. of leukocytes, overview, IC50: 0.0004 mM, kidney enzyme Homo sapiens
3.4.11.2 bradykinin inhibition is enhanced by substance P Homo sapiens
3.4.11.2 iodoacetamide IC50: 0.098 mM, kidney enzyme Homo sapiens
3.4.11.2 additional information structural features of natural peptide hormone inhibitors, overview Homo sapiens
3.4.11.2 morphiceptin
-
 Homo sapiens
3.4.11.2 PAQ-22 IC50: 0.00029 mM, kidney enzyme Homo sapiens
3.4.11.2 phebestin IC50: 0.000015 mM, kidney enzyme Homo sapiens
3.4.11.2 probestin IC50: 0.000016 mM, kidney enzyme Homo sapiens
3.4.11.2 puromycin IC50: 0.0031 mM, kidney enzyme Homo sapiens
3.4.11.2 ranatachykinin
-
 Homo sapiens
3.4.11.2 RB3014 IC50: 0.000025 mM, kidney enzyme Homo sapiens
3.4.11.2 Substance P
-
 Homo sapiens
3.4.11.2 substance P1-4
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.2 cell surface ectoenzyme, the enzyme has an N-terminal membrane anchor Homo sapiens 9986
-
3.4.11.2 membrane type II transmembrane protein expressed on the cell suface Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.2 Zn2+ zinc-metallopeptidase Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.11.2 additional information Homo sapiens mechanism of APN signaling, enzyme inhibition has anti-proliferative effect and causes strong immunosuppression both in vitro and in vivo including growth arrest, induction of immunosuppressive cytokines, e.g. TGF-beta1, reduced expression of inflammatory or T-cell stimulating cytokines, e.g. interleukin-2 and interleukin-12, and mosulation of T-cell signalling pathways, overview, catalytic mechanism, ligand binding structure, active site structure, dynamic opening and closing ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.2 Homo sapiens
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.4.11.2 glycoprotein
-
 Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.11.2 B-cell leukemia cell
-
 Homo sapiens
-
3.4.11.2 epithelial cell
-
 Homo sapiens
-
3.4.11.2 granulocyte
-
 Homo sapiens
-
3.4.11.2 H9 cell
-
 Homo sapiens
-
3.4.11.2 intestine
-
 Homo sapiens
-
3.4.11.2 K-562 cell
-
 Homo sapiens
-
3.4.11.2 KARPAS-299 cell
-
 Homo sapiens
-
3.4.11.2 kidney
-
 Homo sapiens
-
3.4.11.2 leukocyte
-
 Homo sapiens
-
3.4.11.2 macrophage
-
 Homo sapiens
-
3.4.11.2 monocyte
-
 Homo sapiens
-
3.4.11.2 additional information wide tissue distribution, expression analysis, detailed overview Homo sapiens
-
3.4.11.2 myelocytic leukemia cell
-
 Homo sapiens
-
3.4.11.2 P-12 cell
-
 Homo sapiens
-
3.4.11.2 pericardial fluid
-
 Homo sapiens
-
3.4.11.2 synovial fluid
-
 Homo sapiens
-
3.4.11.2 T-lymphocyte activated Homo sapiens
-
3.4.11.2 THP-1 cell
-
 Homo sapiens
-
3.4.11.2 thymocyte
-
 Homo sapiens
-
3.4.11.2 U-937 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.2 L-Ala-4-nitroanilide + H2O
-
 Homo sapiens L-Ala + 4-nitroaniline
-
 ?
3.4.11.2 additional information mechanism of APN signaling, enzyme inhibition has anti-proliferative effect and causes strong immunosuppression both in vitro and in vivo including growth arrest, induction of immunosuppressive cytokines, e.g. TGF-beta1, reduced expression of inflammatory or T-cell stimulating cytokines, e.g. interleukin-2 and interleukin-12, and mosulation of T-cell signalling pathways, overview, catalytic mechanism, ligand binding structure, active site structure, dynamic opening and closing Homo sapiens ?
-
 ?
3.4.11.2 additional information enzyme inhibition causes strong immunosuppression both in vitro and in vivo Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.11.2 dimer monomer structure, three-dimensional sructure, and structure modelling using the crystal structure, dynamic opening and closing of the active site Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
3.4.11.2 alanyl-aminopeptidase
-
 Homo sapiens
3.4.11.2 aminopeptidase N
-
 Homo sapiens
3.4.11.2 APN
-
 Homo sapiens
3.4.11.2 CD13
-
 Homo sapiens
3.4.11.2 mAAP
-
 Homo sapiens

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.11.2 0.00044
-
 Substance P
-
 Homo sapiens
3.4.11.2 0.0094
-
 bradykinin
-
 Homo sapiens
3.4.11.2 0.169
-
 morphiceptin
-
 Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
3.4.11.2 0.000015
-
 IC50: 0.000015 mM, kidney enzyme Homo sapiens phebestin
3.4.11.2 0.000016
-
 IC50: 0.000016 mM, kidney enzyme Homo sapiens probestin
3.4.11.2 0.000025
-
 IC50: 0.000025 mM, kidney enzyme Homo sapiens RB3014
3.4.11.2 0.000075
-
 IC50: 0.000075 mM, kidney enzyme Homo sapiens actinonin
3.4.11.2 0.00029
-
 IC50: 0.00029 mM, kidney enzyme Homo sapiens PAQ-22
3.4.11.2 0.0004
-
 inhibits enzyme activity and cell growth, e.g. of leukocytes, overview, IC50: 0.0004 mM, kidney enzyme Homo sapiens bestatin
3.4.11.2 0.0031
-
 IC50: 0.0031 mM, kidney enzyme Homo sapiens puromycin
3.4.11.2 0.098
-
 IC50: 0.098 mM, kidney enzyme Homo sapiens iodoacetamide