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Literature summary extracted from

  • Malle,D.; Itoh, T.; Hashimoto, W.; Murata, K.; Utsumi, S.; Mikami, B.
    Overexpression, purification and preliminary X-ray analysis of pullulanase from Bacillus subtilis strain 168 (2006), Acta Crystallogr. Sect. F, 62, 381-384.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.41 overexpression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.41 hanging dop method, crystals belong to the orthorhombic space group P2(1)2(1)2(1) with unit-cell parameters a = 70.568, b = 127.68, c = 189.25 A. The crystal contains two molecules of pullulanase in the asymmetric unit, with a solvent content of 53.15% Bacillus subtilis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.41 additional information
-
 additional information Km-value for pullulan is 1.284 mg/ml Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.41 81000
-
 x * 81000, SDS-PAGE Bacillus subtilis
3.2.1.41 81100
-
 x * 81100, calculated from sequence Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.41 Bacillus subtilis
-
-
-
3.2.1.41 Bacillus subtilis 168
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.41 recombinant Bacillus subtilis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.41 24.1
-
-
 Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.41 pullulan + H2O
-
 Bacillus subtilis maltotriose + ?
-
 ?
3.2.1.41 pullulan + H2O
-
 Bacillus subtilis 168 maltotriose + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.41 ? x * 81000, SDS-PAGE Bacillus subtilis
3.2.1.41 ? x * 81100, calculated from sequence Bacillus subtilis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.41 40
-
-
 Bacillus subtilis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.41 79
-
 pullulan pH 5.4 Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.41 6
-
-
 Bacillus subtilis