Literature summary extracted from

Sue, M.; Yamazaki, K.; Kouyama, J.; Sasaki, Y.; Ohsawa, K.; Miyamoto, T.; Iwamura, H.; Yajima, S.
Purification, crystallization and preliminary X-ray analysis of a hexameric beta-glucosidase from wheat (2005), Acta Crystallogr. Sect. F, 61, 864-866.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.21	overexpression of His-tagged Glu1b in Escherichia coli as soluble protein	Triticum aestivum

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
3.2.1.21	purified recombinant enzyme complexed with a substrate aglycone, 20°C, hanging drop vapour diffusion method, 0.001 ml of 3.7 mg/ml protein in solution mixed with 0.001 ml reservoir solution containing 10 mM HEPES, pH 7.2, 1 M LiSO4, and 150 mM NaCl, X-ray diffraction structure determination and preliminary analysis at 1.7 A resolution, molecular replacement	Triticum aestivum

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.21	Triticum aestivum	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.2.1.21	recombinant His-tagged Glu1b from Escherichia coli by nickel affinity chelating chromatography, ultrafiltration, and gel filtration	Triticum aestivum

Subunits

EC Number	Subunits	Comment	Organism
3.2.1.21	hexamer	active form	Triticum aestivum

Synonyms

EC Number	Synonyms	Comment	Organism
3.2.1.21	Glu1b	-	Triticum aestivum

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Release 2023.1 (January 2023)