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Literature summary extracted from

  • Koepke, J.; Ma, X.; Fritzsch, G.; Michel, H.; Stockigt, J.
    Crystallization and preliminary X-ray analysis of strictosidine synthase and its complex with the substrate tryptamine (2005), Acta Crystallogr. Sect. D, 61, 690-693.
    View publication on PubMed

Application

EC Number Application Comment Organism
4.3.3.2 medicine anti-arrhythmic ajmaline agents Rauvolfia serpentina
4.3.3.2 synthesis involved in the biosynthesis of almost all plant monoterpenoid indole alkaloids Rauvolfia serpentina

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.3.3.2 cloned with His-tag, expression in Escherichia coli strain M15, after expression and Ni-NTA column removed by DAPase Rauvolfia serpentina

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
4.3.3.2 crystals of free STR1 and co-crystallization of STR1 and tryptamine, 2.95 A and 2.38 A under cryoconditions, crystal growing by hanging drop vapor diffusion, 302-304 K, 0.8 M potassium sodium tartrate tetrahydrate, pH 7.5 - 8.0, multiple wavelength anomalous dispersion method Rauvolfia serpentina

Organism

EC Number Organism UniProt Comment Textmining
4.3.3.2 Rauvolfia serpentina
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.3.3.2 Ni-NTA column, Mono Q column, concentration to 10 mg/ml in 10 mM Tris-HCl pH 8.0 before crystallization Rauvolfia serpentina

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.3.3.2 tryptamine + secologanin
-
 Rauvolfia serpentina 3alpha(S)-strictosidine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.3.3.2 STR1
-
 Rauvolfia serpentina