EC Number | Cloned (Comment) | Organism |
---|---|---|
3.4.11.10 | overexpression in Escherichia coli | Bacillus subtilis |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
3.4.11.10 | purified recombinant enzyme with or without Zn2+, hanging drop vapour diffusion method, method screening, 0.006 ml protein solution against 1 ml reservoir solution, which contains2.0-2.5 M ammonium sulfate, 14-60 days, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution, multiwavelength anomalous diffraction | Bacillus subtilis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.11.10 | extracellular | - |
Bacillus subtilis | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.11.10 | Zn2+ | dinuclear zinc active center | Bacillus subtilis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 46425 | - |
1 * 46425, sequence calculation | Bacillus subtilis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.11.10 | Bacillus subtilis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.11.10 | recombinant enzyme from Escherichia coli by ion exchange chromatography | Bacillus subtilis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.11.10 | L-Leu-4-nitroanilide + H2O | - |
Bacillus subtilis | L-Leu + 4-nitroaniline | - |
? | |
3.4.11.10 | additional information | the enzyme prefers large and hydrophobic amino acids in peptide and protein substrates | Bacillus subtilis | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.11.10 | monomer | 1 * 46425, sequence calculation | Bacillus subtilis |
3.4.11.10 | More | three-dimensional structure analysis | Bacillus subtilis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.11.10 | BSAP | - |
Bacillus subtilis |
3.4.11.10 | extracellular aminopeptidase | - |
Bacillus subtilis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 80 | - |
20 min, purified recombinant enzyme, stable | Bacillus subtilis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.11.10 | 8 | 9 | - |
Bacillus subtilis |