Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Reiland, V.; Fundoiano-Hershcovitz, Y.; Golan, G.; Gilboa, R.; Shoham, Y.; Shoham, G.
    Preliminary crystallographic characterization of BSAP, an extracellular aminopeptidase from Bacillus subtilis (2004), Acta Crystallogr. Sect. D, 60, 2371-2376.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.4.11.10 overexpression in Escherichia coli Bacillus subtilis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.4.11.10 purified recombinant enzyme with or without Zn2+, hanging drop vapour diffusion method, method screening, 0.006 ml protein solution against 1 ml reservoir solution, which contains2.0-2.5 M ammonium sulfate, 14-60 days, X-ray diffraction structure determination and analysis at 2.2-2.5 A resolution, multiwavelength anomalous diffraction Bacillus subtilis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.4.11.10 extracellular
-
Bacillus subtilis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.11.10 Zn2+ dinuclear zinc active center Bacillus subtilis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.11.10 46425
-
1 * 46425, sequence calculation Bacillus subtilis

Organism

EC Number Organism UniProt Comment Textmining
3.4.11.10 Bacillus subtilis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.11.10 recombinant enzyme from Escherichia coli by ion exchange chromatography Bacillus subtilis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.11.10 L-Leu-4-nitroanilide + H2O
-
Bacillus subtilis L-Leu + 4-nitroaniline
-
?
3.4.11.10 additional information the enzyme prefers large and hydrophobic amino acids in peptide and protein substrates Bacillus subtilis ?
-
?

Subunits

EC Number Subunits Comment Organism
3.4.11.10 monomer 1 * 46425, sequence calculation Bacillus subtilis
3.4.11.10 More three-dimensional structure analysis Bacillus subtilis

Synonyms

EC Number Synonyms Comment Organism
3.4.11.10 BSAP
-
Bacillus subtilis
3.4.11.10 extracellular aminopeptidase
-
Bacillus subtilis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.4.11.10 80
-
20 min, purified recombinant enzyme, stable Bacillus subtilis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.11.10 8 9
-
Bacillus subtilis