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Literature summary extracted from

  • Li, Y.H.; Guo, R.; Yin, Q.Y.; Ding, M.; Zhang, S.L.; Xu, G.J.; Zhao, F.K.
    Purification and characterization of two endo-beta-1,4-glucanases from mollusca, Ampullaria crossean (2005), Acta Biochim. Biophys. Sin., 37, 702-708.
    View publication on PubMed

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 27000
-
 x * 27000, endo-beta-1,4-glucanase EG27, SDS-PAGE Ampullaria crossean
3.2.1.4 45000
-
 x * 45000, endo-beta-1,4-glucanase EG45, SDS-PAGE Ampullaria crossean

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Ampullaria crossean
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4
-
 Ampullaria crossean

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.4 gastric juice endo-beta-1,4-glucanase EG27 Ampullaria crossean
-
3.2.1.4 gastric juice endo-beta-1,4-glucanase EG45 Ampullaria crossean
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 carboxymethyl cellulose + H2O endo-beta-1,4-glucanase EG27 shows high preference for Ampullaria crossean ?
-
 ?
3.2.1.4 carboxymethyl cellulose + H2O endo-beta-1,4-glucanase EG45 shows high preference for Ampullaria crossean ?
-
 ?
3.2.1.4 additional information endo-beta-1,4-glucanase EG27 shows no activity with p-nitrophenyl-beta-D-cellobiose, salicin and starch Ampullaria crossean ?
-
 ?
3.2.1.4 additional information endo-beta-1,4-glucanase EG45 shows no activity with p-nitrophenyl-beta-D-cellobiose, salicin and starch Ampullaria crossean ?
-
 ?
3.2.1.4 Sigmacell 101 + H2O low activity, endo-beta-1,4-glucanase EG27 Ampullaria crossean ?
-
 ?
3.2.1.4 Sigmacell 101 + H2O low activity, endo-beta-1,4-glucanase EG45 Ampullaria crossean ?
-
 ?
3.2.1.4 xylan + H2O low acrtivity, endo-beta-1,4-glucanase EG27 Ampullaria crossean ?
-
 ?
3.2.1.4 xylan + H2O low acrtivity, endo-beta-1,4-glucanase EG45 Ampullaria crossean ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 27000, endo-beta-1,4-glucanase EG27, SDS-PAGE Ampullaria crossean
3.2.1.4 ? x * 45000, endo-beta-1,4-glucanase EG45, SDS-PAGE Ampullaria crossean

Synonyms

EC Number Synonyms Comment Organism
3.2.1.4 endo-beta-1,4-glucanase EG27
-
 Ampullaria crossean
3.2.1.4 endo-beta-1,4-glucanase EG45
-
 Ampullaria crossean

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 50
-
 substrate: carboxymethyl cellulose, endo-beta-1,4-glucanase EG45 Ampullaria crossean
3.2.1.4 50 60 substrate: carboxymethyl cellulose, endo-beta-1,4-glucanase EG27 Ampullaria crossean

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 50
-
 24 h, more than 90% loss of activity, endo-beta-1,4-glucanase EG45 Ampullaria crossean
3.2.1.4 60
-
 24 h, 15% loss of activity, endo-beta-1,4-glucanase EG27 Ampullaria crossean

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 4.4 4.8 substrate: carboxymethyl cellulose, endo-beta-1,4-glucanase EG27 Ampullaria crossean
3.2.1.4 5.5
-
 substrate: carboxymethyl cellulose, endo-beta-1,4-glucanase EG45 Ampullaria crossean

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.4 3 11 24 h, 30°C, stable, endo-beta-1,4-glucanase EG27 Ampullaria crossean
3.2.1.4 5 8 stable at, endo-beta-1,4-glucanase EG45 Ampullaria crossean