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Literature summary extracted from

  • De Cima, S.; Rua, J.; Perdiguero, E.; del Valle, P.; Busto, F.; Baroja-Mazo, A.; de Arriaga, D.
    An acetyl-CoA synthetase not encoded by the facA gene is expressed under carbon starvation in Phycomyces blakesleeanus (2005), Res. Microbiol., 156, 663-669.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.2.1.13 0.6
-
 acetate 30°C, isoenzyme ACS1 Phycomyces blakesleeanus
6.2.1.13 1.02
-
 acetate pH 8.0, 37°C, isoenzyme ACS2 Phycomyces blakesleeanus
6.2.1.13 5.2
-
 propionate pH 8.0, 37°C, isoenzyme ACS2 Phycomyces blakesleeanus
6.2.1.13 8.55
-
 propionate 30°C, isoenzyme ACS1 Phycomyces blakesleeanus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.2.1.13 Ca2+ activates, 45% of the efficiency of Mg2+ and Mn2+ Phycomyces blakesleeanus
6.2.1.13 Co2+ activates, 90% of the efficiency of Mg2+ and Mn2+ Phycomyces blakesleeanus
6.2.1.13 Mg2+ activates Phycomyces blakesleeanus
6.2.1.13 Mn2+ activates Phycomyces blakesleeanus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.2.1.13 70000
-
 x * 70000, isoenzyme ACS1, SDS-PAGE Phycomyces blakesleeanus
6.2.1.13 77000
-
 x * 77000, isoenzyme ACS2, SDS-PAGE Phycomyces blakesleeanus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.2.1.13 additional information Phycomyces blakesleeanus ACS1, encoded by the gene facA, is induced by acetate and repressed by glucose at the transcriptional level ?
-
 ?
6.2.1.13 additional information Phycomyces blakesleeanus ACS2 may be a stress protein, expressed under carbon source starvation ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.2.1.13 Phycomyces blakesleeanus
-
 enzyme form ACS1
-
6.2.1.13 Phycomyces blakesleeanus
-
 enzyme form ACS2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.2.1.13 partial, isoenzyme ACS1 Phycomyces blakesleeanus
6.2.1.13 partial, isoenzyme ACS2 Phycomyces blakesleeanus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
6.2.1.13 mycelium
-
 Phycomyces blakesleeanus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.2.1.13 ATP + acetate + CoA
-
 Phycomyces blakesleeanus ADP + phosphate + acetyl-CoA
-
 ?
6.2.1.13 ATP + propionate + CoA
-
 Phycomyces blakesleeanus ADP + phosphate + propionyl-CoA
-
 ?
6.2.1.13 additional information ACS1, encoded by the gene facA, is induced by acetate and repressed by glucose at the transcriptional level Phycomyces blakesleeanus ?
-
 ?
6.2.1.13 additional information ACS2 may be a stress protein, expressed under carbon source starvation Phycomyces blakesleeanus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.2.1.13 ? x * 70000, isoenzyme ACS1, SDS-PAGE Phycomyces blakesleeanus
6.2.1.13 ? x * 77000, isoenzyme ACS2, SDS-PAGE Phycomyces blakesleeanus

Synonyms

EC Number Synonyms Comment Organism
6.2.1.13 ACS1
-
 Phycomyces blakesleeanus
6.2.1.13 ACS2
-
 Phycomyces blakesleeanus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
6.2.1.13 25 30 isoenzyme ACS1 Phycomyces blakesleeanus
6.2.1.13 50
-
 isoenzyme ACS2 Phycomyces blakesleeanus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
6.2.1.13 20 40 20°C: about 75% of maximal activity, 40°C: about 45% of maximal activity, isoenzyme ACS1 Phycomyces blakesleeanus
6.2.1.13 25 55 25°C: about 45% of maximal activity, 55°C: about 40% of maximal activity, isoenzyme ACS2 Phycomyces blakesleeanus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
6.2.1.13 7.5 8
-
 Phycomyces blakesleeanus